b'Next-Generation Sequencing (NGS) | Research Use Only (RUO) AssaysBioinformatics Software Next-Generation Sequencing (NGS) | RUO Assays Bioinformatics Software Next-Generation Sequencing (NGS) | RUO AssaysLymphoTrack Bioinformatics SoftwareSequencing SummaryUsing the merged read summary interpretation is quick and easy.Software UseThe LymphoTrack Bioinformatics Software package is provided with1 Easy identification of specific types of gene rearrangements such as IGHV3-21.each LymphoTrack Assay to analyze raw FASTQ files for clonality analysis of single or multiple target data sets (IGHV Leader (Leader), IGH FR1, IGH FR2, IGH FR3, IGK, TRG, TRB). For data generated with LymphoTrack IGHV Leader or IGH FR1 Assays the software providesMerge% TotalCumulative Mutation rate In-frameNo stop additional information, including the rate of somatic hypermutationRank Sequence Length count V-gene J-gene reads %partial V-gene(Y/N) codon V-coverage(SHM) and whether a clone will be functional based upon the(%) (Y/N)presence of a premature stop codon. The software can also predict1 TTCTCGTGGTG 455 29603 IGHV4-59_08 IGHJ4_02 9.93 9.93 11.26 Y Y 98.63whether an open reading frame would be in- or out-of-frame, so no external data analysis is required for sample interpretation. 2 CTCGCCCTCCT 463 205 IGHV5-51_01 IGHJ4_02 0.07 9.99 0.00 Y Y 99.66The provided software is composed of two distinct parts:3 GGTTTTCCTTG 484 201 IGHV3-7_01 IGHJ4_02 0.07 10.06 7.77 Y Y 100.001.A bioinformatics Data Analysis Application4 CTCGCCCTCCT 463 185 IGHV5-51_01 IGHJ5_02 0.06 10.12 6.08 Y Y 99.322. Microsoft Excel Data Visualization spreadsheets for each gene5 CTCGCCCTCCT 469 170 IGHV5-51_01 IGHJ4_02 0.06 10.18 0.00 Y Y 99.32target and automated Sample-to-PDF Reports for streamlined 6 CTCGCCCTCCT 466 160 IGHV5-51_01 IGHJ4_02 0.05 10.23 0.00 Y Y 99.66data analysis. 7 CTGCTGCTGAC 460 159 IGHV2-5_10 IGHJ5_02 0.05 10.29 8.08 Y Y 97.648 GGTTTTCCTTG 493 156 IGHV3-48_02 IGHJ6_02 0.05 10.34 3.72 Y Y 98.999 CTCGCCCTCCT 334 153 IGHV5-51_02 IGHJ2_01 0.05 10.39 3.72 Y N 27.7010 CTCGCCCTCCT 334 152 IGHV5-51_02 IGHJ2_01 0.05 10.44 3.38 Y N 26.01Sequence Frequency Graph2 Identification of clonal sequences for follow upSHM rate and indicators to determine LymphoTrack IGH Assay - V - J Sequence Frequencies tracking with LymphoTrack MRD Software. 3 whether a clone is productive.Only 6 LymphoTrack IGH Assay -V J Sequence Frequencies provided for IGHV Leader and IGH FR1. 5 Unique sequenceThe read summary provides sequences from a sample ranked from most abundant to least prevalent. The total read count for individual CATCTGGATACACCTTCACCAGCTACTATATGCACTGGGTGCGACAGGCCCCTGGACAAGGsequences is provided and no independent analysis is required to determine V and J gene families and predictions for SHM when analyzing data GCTTGAGTGGATGGGAATAATCAACCCTAGTGGTGGTAGCACAAGCTACGCACAGAAGTTCC 4 AGGGCAGAGTCACCATGACCAGGGACACGTCCACGAGCACAGTCTACATGGAGCTGAGCAGfrom LymphoTrack IGHV Leader or IGH FR1 Assays. Additionally, the software provides raw and merged data in which reads that differ by 1-2 bp CCTGAGATCTGAGGACACGGCCGTGTATTACTGTGCTAGAGATCTCACAGGTTGTATTAGTare automatically merged to account for possible sequencing errors and to improve the accuracy and ease of sample interpretation. combined.ACCAGCTGCTATCCTCCGAACTACTTTGACTACTGGGGCCAGGGAACCCT% Total Reads % Total Reads3 Gene rearrangement familyIGHV1-46_03 -IGHJ4_02 Ordering Information2 Catalog # Products Quantity Components7-500-0009 LymphoTrack Software- MiSeq 1 CD complimentary with kit purchase1 7-500-0007 LymphoTrack Software - S5/PGM 1 CD complimentary with kit purchase 0 RUO products are for Research Use Only; not intended for in vitro diagnostic use.The stacked bar graph depicts the top 200 sequencing reads for a sample. Each individual colored bar represents a unique population of cells. Different colors stacked at the same point on the x-axis represent unique sequences that utilize the same V and J gene families. The amplicons of these products vary in sequence and may also vary in product size. 52Invivoscribe 2021|53'