b'Next-Generation Sequencing (NGS) | Research Use Only (RUO) AssaysIGH Assay Next-Generation Sequencing (NGS) | RUO Assays IGH Assay Next-Generation Sequencing (NGS) | RUO AssaysLymphoTrack IGH FR1/FR2/FR3 AssaysV-J Sequence Frequencies. The LymphoTrack Software provides a stacked bar graph depicting the relative frequencies for the 200 most Assay Uses allows sequences to be tracked in subsequent samples. Please seeprevalent V H -J Hrearrangements identified in a sample. To learn more the LymphoTrack MRD software section to learn how the LymphoTrackabout the LymphoTrack software, please refer to the LymphoTrack These research use only (RUO) assays for next-generation sequencingAssays can be applied to MRD studies, or emailBioinformatics Software section.(NGS), identify clonal IGH V H -J Hrearrangements, the associated V H -J H marketing@invivoscribe.com.region DNA sequences, the frequency distribution of V Hregion and J Hregion segment utilization. The LymphoTrack FR1 can also identify thePositive clonal (SHM negative) and negative polyclonal DNA controls degree of somatic hypermutation (SHM) of rearranged genes using theare included in kits. A clonal SHM positive control can be purchased Illumina MiSeq, Thermo Fisher Scientific Ion PGM or Ion S5. Theseparately (4-088-0008).LymphoTrack IGH FR1, IGH FR2, and IGH FR3 Assays contain primers that target the conserved framework 1 (FR1), framework 2 (FR2), andBackground framework 3 (FR3) regions, respectively. The LymphoTrack IGH FR1/2/3 Assay kits contain the master mixes of all three frameworks. The human immunoglobulin heavy chain (IGH) gene locus on chromosome 14 (14q32.3) includes 46-52 functional and 30 nonfunctional variable (V H ) gene segments, 27 functional diversity (D H ) Summary and Explanation of the TestHgene segments, and 6 functional joining (J ) gene segments spread The LymphoTrack IGH Assays represent a significant improvementover 1250 kilobases.over clonality assays that utilize fragment analysis by providing fourDuring development of lymphoid cells, the antigen receptor genes go important and complementary uses: through somatic gene rearrangements. 1For example, during B-cell 1.Detection of clonal populations. development, genes encoding the IGH molecules are assembled from multiple polymorphic gene segments that undergo rearrangementsOrdering Information2. Identification of sequence information and V H -J Hand selection, generating V H -D H -J Hcombinations. Since leukemias Catalog # Products Quantity Componentssegment utilization.and lymphomas originate from the malignant transformation of 3. The LymphoTrack IGH FR1 Assays provide the degree of SHM ofindividual lymphoid cells, all leukemias and lymphomas generally7-121-0129 LymphoTrack IGH FR1/2/3 Assay Kit A - MiSeqIndices 1-8 (5 sequencing reactions each)share one or more cell-specific or clonal antigen receptor gene the immunoglobulin variable heavy chain (IGHV). rearrangements. Therefore, tests that detect IGH clonal populations7-121-0139 LymphoTrackIGH FR1/2/3 Assay Panel - MiSeq Indices 1-24 (5 sequencing reactions each)7-121-0009 LymphoTrackIGH FR1 Assay Kit A - MiSeq Indices 1-8 (5 sequencing reactions each)4. The ability to track sequences in subsequent samples with thecan be useful in the study of B- and T-cell malignancies.Invivoscribe LymphoTrack MRD Software. 7-121-0039 LymphoTrack IGH FR1 Assay Panel - MiSeqIndices 1-24 (5 sequencing reactions each)Each single multiplex master mix targets one of the conserved IGHSpecimen Requirement 7-121-0149 LymphoTrack IGH FR1 Assay Panel B - MiSeqNEW! Indices 25-48 (5 sequencing reaction each)framework regions (FR1, FR2, or FR3) within the V Hand the J Hregions7-121-0089 LymphoTrack IGH FR2 Assay Kit A - MiSeqIndices 1-8 (5 sequencing reactions each)described in lymphoid malignancies. Targeting all three framework50 ng of high-quality genomic DNA.regions significantly reduces the risk of not being able to detect7-121-0099 LymphoTrack IGH FR2 Assay Panel - MiSeqIndices 1-24 (5 sequencing reactions each)the presence of clonality, as somatic hypermutations in the primerReference 7-121-0109 LymphoTrack IGH FR3 Assay Kit A - MiSeqIndices 1-8 (5 sequencing reactions each)binding sites of the involved V Hgene segments can impede DNA amplification. 11.S Tonegawa. Nature 302: 575-581 (1983). 7-121-0119 LymphoTrackIGH FR3 Assay Panel - MiSeq Indices 1-24 (5 sequencing reactions each)7-500-0009 LymphoTrack Software - MiSeq1 CD complimentary with purchasePrimers included in the master mixes are designed with Illumina adapters and indices (8 included in Kit A, 24 included in the Panel, and7-121-0057 LymphoTrack IGH FR1/2/3 Assay - S5/PGM12 + 12 + 12 indices - 5 sequencing reactions eachan independent 24 included in the Panel B) or Thermo Fisher adaptors7-121-0007 LymphoTrack IGH FR1 Assay - S5/PGM12 indices - 5 sequencing reactions eachand indices (12 indices per framework kits). This allows for a one-step PCR reaction to generate sequence-ready amplicons and pooling of7-121-0037 LymphoTrack IGH FR2 Assay - S5/PGM12 indices - 5 sequencing reactions eachseveral different samples on the same Illumina MiSeq cell, Ion S5 or7-121-0047 LymphoTrack IGH FR3 Assay - S5/PGM12 indices - 5 sequencing reactions eachIon PGM chip. The LymphoTrack bioinformatics software enables easy7-500-0007 LymphoTrack Software - S5/PGM1 CD complimentary with purchaseanalysis and visualization of data and the LymphoTrack MRD Software 7-500-0008LymphoTrack MRD Software 1 CD complimentary with purchaseSimple representation of the organization of the immunoglobulin heavy chain (IGH) D H J H gene locus on chromosome 14. Depicted are the variable region (V H ) genes and V L V H -FR1 V H -FR2 V H -FR3J Hprimersdownstream consensus joining region genes segments (J H ) that are involved in rearrangements. V Hfamily primers42Invivoscribe 2021|43'