b'Next-Generation Sequencing (NGS) | Research Use Only (RUO) AssaysTRB Assay Next-Generation Sequencing (NGS) | RUO Assays TRB Assay Next-Generation Sequencing (NGS) | RUO AssaysLymphoTrack TRB AssayReagents - MiSeq DetectionAssay Uses BackgroundKit A Components Panel Components(includes all master mixes from Kit A plus the items below)This research use only (RUO) assay for next-generation sequencingThe LymphoTrack TRB Assay represent a significant improvement overMaster Mix Name Index # Master Mix Name Index # Master Mix Name Index #(NGS) identifies clonal TRB V-(D-)J rearrangements, the associatedclonality assessment by fragment analysis by providing two important V-(D-)J region DNA sequences, and provides the frequencyand complementary uses:TRB MiSeq 01 A001 TRB MiSeq 09 A009 TRB MiSeq 18 A018distribution of V, D, and J region segment utilization using theTRB MiSeq 02 A002 TRB MiSeq 10 A010 TRB MiSeq 19 A019Illumina MiSeq platform.1. Detection of clonal populations.TRB MiSeq 03 A003 TRB MiSeq 11 A011 TRB MiSeq 20 A0202. Identification of sequence information and gene segment utilization. Analysis of the rearranged TRB locus increases the probability 3. Ability to track sequences in subsequent samples with the use of theTRB MiSeq 04 A004 TRB MiSeq 12 A012 TRB MiSeq 21 A021of identifying T-cell receptor gene rearrangements, as compared Invivoscribe MRD Software.TRB MiSeq 05 A005 TRB MiSeq 13 A013 TRB MiSeq 22 A022to testing for TRG gene rearrangements only. As a result, combining the analysis of TRB and TRG loci increases the sensitivity TRB MiSeq 06 A006 TRB MiSeq 14 A014 TRB MiSeq 23 A023of clonality detection.The human T-cell receptor beta (TRB) gene locus on chromosomeTRB MiSeq 07 A007 TRB MiSeq 15 A015 TRB MiSeq 25 A0257 (7q34) includes 65 V (variable) gene segments, followed by two separate clusters of genes each containing a D (diversity) gene,TRB MiSeq 08 A008 TRB MiSeq 16 A016 TRB MiSeq 27 A027several J (joining) genes, and a C (constant) region spread over Summary and Explanation of the TestControls Controls685 kilobases. The two Cgenes, TRBC1 and TRBC2, encode highlyTRB POS (+) Qty. 1 NGS NEG (-) Qty. 1 TRB POS (+) Qty. 3 NGS NEG (-) Qty. 3This assay utilizes a single multiplex master mix to target conserved homologous products with no functional difference.V, D and J regions of the human TRB gene locus described in lymphoid malignancies, thereby reducing sample DNA requirements andDuring lymphoid cell development, antigen receptor genes simplifying the testing workflow. undergo somatic gene rearrangements. 1Specifically, during T-cell development, genes encoding TRB molecules are assembled The LymphoTrack TRB master mix primers are also designed withfrom multiple polymorphic gene segments that generate V-D-J Illumina adapters and 8 indices (Kit A) or 24 indices (Panel). combinations unique in both length and sequence. 2This allows amplicons generated from different indexed TRB V-J Sequence Frequencies. The LymphoTrack bioinformatics software Since leukemias and lymphomas originate from the malignantprovides PDF reports which include Top 10 Merged Read Summary as well master mixes to be pooled into a single sequencing library. transformation of individual lymphoid cells, all leukemias andas a stacked bar graph depicting the relative frequencies of the 200 most prevalent rearrangements sequenced and identified in the sample. To learn The associated LymphoTrack Software is capable of sorting lymphomas generally share one or more cell-specific or clonalmore about the LymphoTrack software, please refer to the LymphoTrack complex NGS data by gene target. This offers a second layer antigen receptor gene rearrangements. Therefore, tests that detectBioinformatics Software section.of multiplexing to reduce per sample testing costs by allowingTRB clonal rearrangements can be useful in the study of B- andamplicons from any LymphoTrack Assay (e.g. IGH, IGK, TRB, TRG)T-cell malignancies.to be sequenced on the same flow cell. In addition, the LymphoTrack Software provides easy visualization of data and the LymphoTrackNote: For a more thorough explanation of the locus and the targeted MRD Software allows identified sequences to be tracked anddeep sequencing strategy, please refer to Principle of Immunoglobulin monitored in subsequent samples. and T-Cell Receptor Gene Rearrangement. 2 Positive clonal and negative polyclonal DNA controls are included in kits. Please see the LymphoTrack MRD software section to learn howSpecimen Requirementthe LymphoTrack Assays can be applied to MRD studies, or email 50 ng of high-quality genomic DNA.marketing@invivoscribe.com.References Ordering InformationCatalog # Products Quantity Components1. Tonegawa, S. (1983). Nature 302, 575-581. 7-225-0009 LymphoTrack TRB Assay Kit A - MiSeq8 indices - 5 sequencing reactions each2. Miller JE. (2013) Molecular Genetic Pathology(2nd Edition.,sections 30.2.7.13 and 30.2.7.18). 7-225-0019 LymphoTrack TRB Assay Panel - MiSeq24 indices - 5 sequencing reactions each7-500-0009 LymphoTrack Software - MiSeq1 CD complimentary with purchase7-500-0008 LymphoTrack MRD Software* 1 CD complimentary with purchase*MRD Software can be used to track sequences generated by either LymphoTrack Assays - MiSeq or Ion S5/PGM. Simplified Representation of the TRB GeneRUO products are for Research Use Only; not intended for in vitro diagnostic use.V D1 J1 C1 D2 J2 C2Depicted are the variable (V), diversity (D), and joining (J) gene regions involved in TRB gene rearrangements, in addition to the downstream consensus (C) gene regions. 50Invivoscribe 2021|51'