b'Gel & Capillary | RUO AssaysGel and Capillary Gel and CapillaryTCRD Gene Clonality AssaysResearch Use Only (RUO) Assay TCRD Gene Clonality AssaysResearch Use Only (RUO) AssayTCRD Gene Clonality AssaysAssay Use rearranged TRD locus. This limited germline diversity encoded at the TRD locus in conjunction with extensive junctional diversity results in a T-Cell receptor delta (TCRD)gene clonality assays are useful for theuseful target for PCR analysis. TRD recombination events have beenReagentsidentification of T-cell clonality, studying clonal T-cell populations, andused most extensively as clonal markers in both T- and B-cell ALL. This evaluation of new research and methods in malignancy studies. standardized multiplex PCR assay detects the vast majority of clonalControls Concentration Units in Assay Units in Assay MegaKitTRD gene rearrangements using a single multiplex master mix 1 .IVS-0021 Clonal Control DNA 200 g/mL 1 x 100 L tube 5 x 100 L tubesSummary and Explanation of the Test IVS-0000 Polyclonal Control DNA 200 g/mL 1 x 100 L tube 5 x 100 L tubesThe TCRD Tube Master Mix targets conserved regions withinSpecimen Requirements Master Mixes Target Units in Assay Units in Assay MegaKitthe variable (V1-6), the diversity (D2-3) and the joining (J1-4) regions that flank the unique, hypervariable, antigen-binding,This assay tests extracted and purified genomic DNA (gDNA). TCRD Tube Multiple V + D + J1 x 1500 L tube 10 x 1500 L tubescomplementarity determining region 3 (CDR3) of the T-cell receptorSpecimen Control Size Ladder Multiple Genes 1 x 1500 L tube 10 x 1500 L tubesdelta (TRD, formerly known as TCRD). Positive and negative controls, as well as a Specimen Control Size Ladder Master Mix, areReferencesincluded. PCR products can be analyzed by capillary electrophoresis1.FL Lavender et al., Leukemia 17: 2292-2296 (2003).or heteroduplex analysis. Clonality is indicated if the master mix2. JJM van Dongen et al., Leukemia 17: 2257-2317 (2003).generates clonal products. Ordering InformationBackgroundCatalog # Products Quantity1-206-0010 TCRD Gene Clonality Assay - Gel Detection 33 reactionsThe human T-cell receptor delta (TRD, formerly known as TCRD) gene locus is comprised of a cluster of 10 genes located on chromosome1-206-0020 TCRD Gene Clonality Assay MegaKit - Gel Detection 330 reactions14 (14q11.2) spread over 60 kilobases, localized between the T-cell1-206-0011 TCRD Gene Clonality Assay - ABI Fluorescence Detection 33 reactionsreceptor alpha (TRA, formerly known as TCRA) variable (V) and joining (J) gene segments. It is made up of eight variable (V),1-206-0021TCRD Gene Clonality Assay MegaKit - ABI Fluorescence Detection 330 reactionsthree diversity (D), and four joining (J) gene segments. 1At least five of the eight V gene segments can also rearrange to J gene segments and other V gene segments may also be utilized in TRD gene rearrangements in rare cases. Although the small number of V, D, and J gene segments available for recombination limits the potential combinatorial diversity, the complementarity determining region 3 (CDR3) or junctional diversity is extensive due to the addition of N regions, P regions, and random deletion of nucleotides by recombinases. This diversity is also extended by the recombination of up to three D segments and therefore up to four N regions within the This assay is based on the EuroClonality/BIOMED-2 Concerted Action BMH4-CT98-3936.Figure Legend: Simplified diagram of a representative rearranged T-cell receptor delta gene showing the approximate placement of the upstream and downstream DNA primers. The numbers of primers and their specificity are listed for the TRD Tube Master Mix tube.112Invivoscribe 2021|113'