b'Gel & Capillary | RUO AssaysGel and Capillary Gel and CapillaryTCRB Gene Clonality AssaysResearch Use Only (RUO) Assay TCRB Gene Clonality Assays Research Use Only (RUO) AssayTCRB Gene Clonality AssaysAssay Use Specimen Requirements ReagentsT-Cell receptor beta (TCRB)gene clonality assays are useful for theThis assay tests extracted and purified genomic DNA (gDNA). Controls Concentration Units in Assay Units in Assay MegaKitidentification of T-cell clonality, studying clonal T-cell populations, and evaluation of new research and methods in malignancy studies. IVS-0009 Clonal Control DNA 200 g/mL 1 x 100 L tube 5 x 100 L tubesReferences IVS-0004 Clonal Control DNA 200 g/mL 1 x 100 L tube 5 x 100 L tubesSummary and Explanation of the Test1.M Bruggemann et al., Leukemia 17: 2283-2289 (2003). IVS-0021 Clonal Control DNA 200 g/mL 1 x 100 L tube 5 x 100 L tubesThree multiplex master mixes target conserved regions within the2. JJM van Dongen et al., Leukemia 17: 2257-2317 (2003). IVS-0000 Polyclonal Control DNA 200 g/mL 1 x 100 L tube 5 x 100 L tubesvariable (V), diversity (D), and the joining (J) regions that flankMaster Mixes Target Units in Assay Units in Assay MegaKitthe unique hypervariable, antigen-binding, complementarity determining region 3 (CDR3) of the T-cell receptor beta locus. TCRB Tube A Multiple V + J1/2 1 x 1500 L tube 10 x 1500 L tubesTube A contains 23 V primers, six J1 primers, and three J2 primers. TCRB Tube B Multiple V + J2 1 x 1500 L tube 10 x 1500 L tubesTube B contains 23 V and four J2 primers. Tube C contains two D and 13 J primers. Positive and negative DNA controls, as well TCRB Tube C Multiple D + J1/2 1 x 1500 L tube 10 x 1500 L tubesas a Specimen Control Size Ladder Master Mix, are included.PCR products can be analyzed by capillary electrophoresisor heteroduplex analysis. Clonality is indicated, if any one of the master mixes generates clonal products.Ordering InformationBackgroundCatalog # Products QuantityThe human T-cell receptor beta (TRB, formerly known as TCRB) gene1-205-0010 TCRB Gene Clonality Assay - Gel Detection 33 reactionslocus on chromosome 7 (7q34, formerly 7q35) includes 64-67 variable1-205-0020 TCRB Gene Clonality Assay MegaKit - Gel Detection330 reactions(V) gene segments (belonging to 30 subgroups), two diversity (D)1-205-0011 TCRB Gene Clonality Assay - ABI Fluorescence Detection 33 reactionsgene segments, and 13 joining (J) gene segments, spread over 685 kilobases. The diversity of this locus has complicated PCR-based1-205-0021TCRB Gene Clonality Assay MegaKit - ABI Fluorescence Detection 330 reactionstesting and extended dependence on Southern blot analysis in many testing centers. However, this standardized multiplex PCR assay detects the vast majority of clonal TRB gene rearrangements using only three multiplex master mixes. 1This assay is based on the EuroClonality/BIOMED-2 Concerted Action BMH4-CT98-3936.Figure Legend: Simplified diagram of a representative rearranged T-cell receptor beta gene showing the approximate placement of the upstream and downstream DNA primers. The numbers of primers and their specificity are listed for Master Mix Tubes A, B, and C.106Invivoscribe 2021|107'