b'Gel & Capillary | RUO AssaysGel and Capillary Gel and CapillaryIGK Gene Clonality AssaysResearch Use Only (RUO) Assay IGK Gene Clonality AssaysResearch Use Only (RUO) AssayIGK Gene Clonality AssaysAssay Useencode the first 95 N-terminal amino acids. Positions 96-108 are Immunoglobulin kappa light chain (IGK) gene clonality assays areencoded by one of five joining (J) gene segments. The constant (C) useful for the identification of B-cell clonality, studying clonal B-cellportion of the kappa light chain (amino acids 109-214) is encodedpopulations, and evaluation of new research and methods by a single constant (C) region separated from the J regionReagentsin malignancy studies. by an intron. The length of the hypervariable complementarity determining region 3 (CDR3) in kappa light chain genes is limitedControls Concentration Units in Assay Units in Assay MegaKitand rearrangements in this region display significant skewing Summary and Explanation of the Test2 IVS-0007 Clonal Control DNA 200 g/mL 1 x 100 L tube 5 x 100 L tubes(platykurtosis).Two master mixes target conserved regions within the variable IVS-0000 Polyclonal Control DNA 200 g/mL 1 x 100 L tube 5 x 100 L tubes(V1-7) and the joining (J1-5) regions that flank the uniqueTherefore, clonal CDR3 products generated from this region areMaster Mixes Target Units in Assay Units in Assay MegaKithypervariable, antigen-binding, complementarity determiningmost easily and reliably identified by heteroduplex analysis using region 3 (CDR3). Other primers target the K deand intragenic regions.standard polyacrylamide gels. Alternatively, capillary electrophoresisIGK Tube A V- J 1 x 1500 L tube 10 x 1500 L tubesor gene sequencing instruments coupled with differential fluorescenceIGK Tube B V-K de 1 x 1500 L tube 10 x 1500 L tubesTube A contains six upstream primers and two J region primers.detection can be used for analysis.Tube B contains six upstream V region primers, an upstreamSpecimen Control Size Ladder Multiple Genes 1 x 1500 L tube 10 x 1500 L tubesintragenic primer and a downstream K deprimer. Positive and negative controls, as well as a Specimen Control Size Ladder MasterSpecimen RequirementsMix, are included. PCR products can be analyzed by capillary electrophoresis or heteroduplex analysis. Clonality is indicated if anyThis assay tests extracted and purified genomic DNA (gDNA). Ordering Informationone of the master mixes generates clonal products.Catalog # Products QuantityBackgroundReferences 1-102-0020 IGK Gene Clonality Assay - Gel Detection 33 reactionsThe human immunoglobulin kappa (IGK) light chain locus on the1.AW Langerak et al., Leukemia 17: 2275-2280 (2003). 1-102-0030 IGK Gene Clonality Assay MegaKit - Gel Detection 330 reactions2. EP Rock, PR Sibbald, MM Davis, and YH Chien. J. Exp. Med. 179(1):1-102-0021 IGK Gene Clonality Assay - ABI Fluorescence Detection 33 reactionsshort arm of chromosome 2 (2p12, formerly 2p11.2) spans 1820 kb. It is made up of 76 variable (V) gene segments belonging to 7 subgroups,323-328 (1994).5 joining (J) gene segments, and one constant (C) gene segment.3. JJM van Dongen et al., Leukemia 17: 2257-2317 (2003). 1-102-0031 IGK Gene Clonality Assay MegaKit - ABI Fluorescence Detection 330 reactionsProductive assembly of the kappa gene is successful in about 60% of human B lymphocytes. 1However, even when unsuccessful, clonal B cells generally retain the rearranged kappa genes. The V segments This assay is based on the EuroClonality/BIOMED-2 Concerted Action BMH4-CT98-3936.Figure Legend: Schematic diagram of the immunoglobulin kappa light chain gene complex on chromosome 2p11.2. Shown are the relative positions and orientations for the V-J, and K deprimers, which are included in the IGK master mix tubes.102Invivoscribe 2021|103'