b'Gel & Capillary | RUO AssaysGel and Capillary Gel and CapillaryBCL2/J Ht(14;18) Translocation AssayResearch Use Only (RUO) Assay BCL2/JH t(14;18) Translocation AssayResearch Use Only (RUO) AssayBCL2/Jt(14;18) Translocation AssayHReagentsAssay Use Specimen Requirements Controls Concentration Units in Assay Units in Assay MegaKitThis Research Use Only assay identifies BCL2/J Ht(14;18) translocations. This assay tests extracted and purified genomic DNA (gDNA). IVS-0030 Clonal Control DNA200 g/mL 1 x 100 L tube 5 x 100 L tubesIVS-0031 Clonal Control DNA 200 g/mL 1 x 100 L tube 5 x 100 L tubesSummary and Explanation of the TestReferences IVS-0009 Clonal Control DNA 200 g/mL 1 x 100 L tube 5 x 100 L tubesFive master mixes are included in this assay kit. Two master mixesMaster Mixes Target Units in Assay Units in Assay MegaKittarget BCL2 major break point (MBR) translocations and two target1.MS Lee et al., Science 237: 175-178 (1987). BCL2/J Ht(14;18) (MBR) Mix 1b Inside BCL2 MBR 1 x 1500 L tube 10 x 1500 L tubesBCL2 minor cluster region (mcr) translocations. An Amplification2. M Crescenzi et al., Proc. Natl. Acad. Sci. USA 85: 4869-4873 (1988).Control Master Mix is also included to ensure the quality and quantityBCL2/J Ht(14;18) (mcr) Mix 2b Inside BCL2 mcr 1 x 1500 L tube 10 x 1500 L tubesof sample DNA. Positive and negative controls are also included.BCL2/J Ht(14;18) (MBR) Mix 1a Outside BCL2 MBR 1 x 1500 L tube 10 x 1500 L tubesThis assay can be run either in a standard or nested assay format.BCL2/J Ht(14;18) (mcr) Mix 2a Outside BCL2 mcr 1 x 1500 L tube 10 x 1500 L tubesPCR products can be analyzed by standard gel electrophoresis with ethidium bromide staining. A BCL2 translocation is indicated if just oneAmplification Control HLA-DQa 1 x 1500 L tube 10 x 1500 L tubesof the 2 ndround master mixes (mixes ending in b) generates product(s) within the valid size range.BackgroundOrdering InformationBCL2 translocations are reciprocal chromosome exchanges thatCatalog # Products Quantityplace the bcl-2 proto-oncogene, located on chromosome 18, under aberrant transcriptional control of the immunoglobulin heavy chain1-309-0010 BCL2/J Ht(14;18) Translocation Assay - Gel Detection 30 reactionsgene, located on chromosome 14.The bcl-2 protein is an antagonist to apoptosis (programmed cell death), a normal process designed to eliminate unneeded and damaged cells during hematopoiesis.Increased expression of the bcl-2 protein leads to an increase in the levels of B-cells in the body. Figure Legend: Simplified view of the genomic organization of theBCL2 GeneBCL2 and IGH genes on chromosomes 18 and 14, respectively. YellowChromosome 18q21boxes represent the exon regions of the BCL2 gene. Exons of the immunoglobulin heavy chain gene are represented in other colors. The solid black lines represents intron regions, which have been leftHeavy Chain Geneincompletely spliced to assist in demarcation of the exon segments.Chromosome 14q32MBR and mcr type t(14;18) translocations are shown in the lower portions of the figure with the relative positions of primers and the sizeBCL2/IgH Fusion Geneof the amplicons generated from the positive control DNAs indicated. MBR J HBreakpointsBCL2/IgH Fusion Genemcr J HBreakpoints116Invivoscribe 2021|117'