b'Gel & Capillary | RUO AssaysGel and Capillary Gel and CapillaryT-Cell Receptor Gamma Gene Research Use Only (RUO) Assay T-Cell Receptor Gamma Gene Research Use Only (RUO) AssayRearrangement Assays Rearrangement AssaysT-Cell Receptor Gamma Gene Rearrangement AssaysAssay Use Specimen Requirements ReagentsT-Cell receptor gamma (TCRG)gene clonality assays are useful forThis assay tests extracted and purified genomic DNA (gDNA). Controls Concentration Units in Assay Units in Assay MegaKitthe identification of T-cell clonality, studying clonal T-cell populations, and evaluation of new research and methods in malignancy studies. References IVS-0009 Clonal Control DNA 200 g/mL 1 x 100 L tube 5 x 100 L tubesIVS-0000 Polyclonal Control DNA 200 g/mL 1 x 100 L tube 5 x 100 L tubesSummary and Explanation of the Test1.JE Miller, SS Wilson, DL Jaye, and M Kronenberg. J. Mol. Diag. 4:Master Mixes Target Units in Assay Units in Assay MegaKit 101-117 (1999).Sample genomic DNA is amplified using two master mixes that2. K Beldjord et al., Leukemia 17: 2289-2292 (2003).T-Cell Receptor Gamma Mix 1 V1-8,9 + J1/2 1 x 1500 L tube 10 x 1500 L tubesindependently target conserved regions within the variable (V) andT-Cell Receptor Gamma Mix 2 Alt V + J1/2 1 x 1500 L tube 10 x 1500 L tubesjoining (J) regions that flank the unique, hypervariable, antigen-binding, complementarity determining region 3 (CDR3). This assayAmplification Control HLA-DQa 1 x 1500 L tube 10 x 1500 L tubestargets V1-9 and J gene segments. Positive and negative DNA controls, as well as an internal Amplification Control Master Mix, are included. PCR products can be analyzed by capillary electrophoresis or standard gel electrophoresis with ethidium bromide staining.Ordering InformationBackgroundCatalog # Products QuantityThe T-cell receptor gamma (TRG, formerly known as TCRG) chain1-207-0010 T-Cell Receptor Gene Rearrangement Assay - Gel Detection 30 reactionslocus spans 160 kilobases on chromosome 7 (7p14). The locus consists1-207-0051 T-Cell Receptor Gene Rearrangement Assay - ABI Fluorescence Detection30 reactionsof 14 variable (V) gene segments in six subgroups, and five joining (J) gene segments interspersed between two constant (C) gene segments. However, the repertoire of functional TRG molecules is limited to 4-6 functional V gene segments that belong to two subgroups. 2Rearrangement of the V and J gene segments of the TRG locus results in V-Jproducts of unique length and sequence. Clonal TRG rearrangements can be most rapidly identified by analyzing the size distribution of DNA products amplified from conserved sequences that flank this V-Jregion. 1DNA isolated from a normal heterogeneous population of polyclonal T-cells produces a Gaussian distribution (bell-shaped size curve) of amplified products. DNA amplified from a clonal T-cell population generates one or two product(s) of unique size that reflects proliferation of a single rearranged clone. 1,2Figure Legend: Simplified figure representing the organization of a rearranged T-cell receptor gamma chain gene on chromosome 7. Colored arrows represent conserved regions within the variable region gene segments targeted by primers. Primers are represented by arrows with the size range of valid products generated with each of the master mixes indicated below the figure. Colors correspond to the peak colors assigned to products when differential fluorescence detection methods are used.110Invivoscribe 2021|111'