b'Gel & Capillary | RUO AssaysGel and Capillary Gel and CapillaryBCR/ABL t(9;22) Translocation Assays Research Use Only (RUO) Assay BCR/ABL t(9;22) Translocation AssaysResearch Use Only (RUO) AssayBCR/ABL t(9;22) Translocation AssaysAssay Use Specimen RequirementsThis Research Use Only assay identifies BCR/ABL t(9;22) translocations. This assay tests complementary DNA (cDNA) template. ReagentsSummary and Explanation of the TestReferences Controls Concentration Units in Assay Units in Assay MegaKitThe master mixes are included in these assay kits used to amplify1.R Kurzrock et al., Ann. Intern. Med. 138: 819-30 (2003). IVS-0032 Clonal Control RNA 400 g/mL 1 x 100 L tube 5 x 100 L tubescomplementary DNA (cDNA) produced from specimen(s), and positive2. JV Melo. Blood 88: 2375-2384 (1996). IVS-0011 Clonal Control RNA 400 g/mL 1 x 100 L tube 5 x 100 L tubesand negative RNA controls (included). Primers target an internal3. JP Radich et al., Blood 85: 2632-2638 (1995).control transcript (Abl) and p190-, p210-, and p230-type transcriptsIVS-0035 Clonal Control RNA 400 g/mL 1 x 100 L tube 5 x 100 L tubesexpressed from BCR-ABL1 translocations. Amplicon products can beMaster Mixes Target Units in Assay Units in Assay MegaKitanalyzed by capillary electrophoresis or standard gel electrophoresis with ethidium bromide staining. A BCR-ABL1 translocation is indicatedBCR/ABL t(9;22) Mix 1a Abl1 x 1500 L tube 10 x 1500 L tubesif just one of the 2nd round master mixes (Mix 2b, Mix 2c, Mix 3b, MixBCR/ABL t(9;22) Mix 2a p190 1 x 1500 L tube 10 x 1500 L tubes3c, or Mix 3d) generates product(s) of the valid size. Reagents for RNA extraction and reverse transcription are not included. This assayBCR/ABL t(9;22) Mix 3ap210+230 1 x 1500 L tube 10 x 1500 L tubesis compatible with all standard RNA extraction and cDNA synthesisBCR/ABL t(9;22) Mix 1b Abl 1 x 1500 L tube 10 x 1500 L tubesmethods. This is a qualitative assay and has not been validated for quantitative use. BCR/ABL t(9;22) Mix 2b p190 1 x 1500 L tube 10 x 1500 L tubesBCR/ABL t(9;22) Mix 2c p190 1 x 1500 L tube 10 x 1500 L tubesBackgroundBCR/ABL t(9;22) Mix 3b p210+230 1 x 1500 L tube 10 x 1500 L tubesBCR/ABL translocations are associated with a variety of hematologicBCR/ABL t(9;22) Mix 3c p210+230 1 x 1500 L tube 10 x 1500 L tubesmalignancies. The Philadelphia chromosome (Ph1) is a specificBCR/ABL t(9;22) Mix 3d p210+230 1 x 1500 L tube 10 x 1500 L tubeschromosomal abnormality that results from reciprocal t(9;22)(q34;ql1) chromosomal rearrangements fuse coding regions of the BCR gene, located on chromosome 22, with the ABL receptor-independent tyrosine kinase gene on chromosome 9. This assay detects and identifies the variety of p190-, p210- and p230-type transcriptsOrdering Informationproduced from all known BCR/ABL translocations.Catalog # Products Quantity1-310-0010 BCR/ABL t(9;22) Translocation Assay - Gel Detection 30 reactions1-310-0031 BCR/ABL t(9;22) Translocation Assay - ABI Fluorescence Detection 30 reactionsFigure Legend: This figure shows the genomic organization of the BCR and ABLBCRgenes on chromosomes 22 and 9, respectively. Boxes represent exon regions ofChromosome 22the ABL (red boxes) and BCR encoding exons (other colors). The solid black line represents intron regions, which have been left incompletely spliced to assist in demarcation of the exon segments. The location of exon regions targeted by labeled and unlabeled primers are indicated by arrows. A p210-type BCR-ABL1ABLtranslocation (b3a2 junction) is depicted in the lower portion of the figure alongChromosome 9with the control ABL transcript control.BCR/ABL t(9;22) Translocationp210 Type, b3a2 junctionABL cDNA Control120Invivoscribe 2021|121'