b'Next-Generation Sequencing (NGS) | Research Use Only (RUO) AssaysIGK Assay Next-Generation Sequencing (NGS) | RUO Assays IGK Assay Next-Generation Sequencing (NGS) | RUO AssaysLymphoTrack IGK Assay Reagents - MiSeq DetectionKit A Components Panel Components(includes all master mixes from Kit A plus the items below)Master Mix Name Index # Master Mix Name Index # Master Mix Name Index #IGK MiSeq 01 A001 IGK MiSeq 09 A009 IGK MiSeq 18 A018Assay Uses BackgroundIGK MiSeq 02 A002 IGK MiSeq 10 A010 IGK MiSeq 19 A019This research use only (RUO) assay for next-generation sequencingThe human immunoglobulin kappa (IGK) gene locus on chromosomeIGK MiSeq 03 A003 IGK MiSeq 11 A011 IGK MiSeq 20 A020(NGS), identifies clonal IGK V-J, V-K de , and intron-K de(INTR-K de )2 (2p11.2) includes 76 V (variable) region genes spanning 7 subgroupsIGK MiSeq 04 A004 IGK MiSeq 12 A012 IGK MiSeq 21 A021rearrangements, the corresponding DNA sequences, and providesand 5 J (joining) region gene segments upstream of the C region.IGK MiSeq 05 A005 IGK MiSeq 13 A013 IGK MiSeq 22 A022the distribution frequency of V-J, V-K de , and INTR-K desegmentThe K de , approximately 24 kb downstream of the J-C region, can also utilization using the Illumina MiSeq or Thermo Fisher Scientificrearrange with V gene segments and the isolated recombinationIGK MiSeq 06 A006 IGK MiSeq 14 A014 IGK MiSeq 23 A023Ion PGM and Ion S5 platforms. signal sequence in the J-C intronic region. IGK MiSeq 07 A007 IGK MiSeq 15 A015 IGK MiSeq 25 A025During development of lymphoid cells, antigen receptor genes undergoIGK MiSeq 08 A008 IGK MiSeq 16 A016 IGK MiSeq 27 A027Summary and Explanation of the Testsomatic gene rearrangements. 1Specifically during B-cell development,Controls Controlsgenes encoding IGK molecules are assembled from multipleIGK POS (+) Qty. 1 NGS NEG (-) Qty. 1 IGK POS (+) Qty. 3 NGS NEG (-) Qty. 3The LymphoTrack IGK Assay represents a significant improvementpolymorphic gene segments that undergo rearrangements generating over clonality assays that utilize fragment analysis by providing threeV-J combinations unique in both length and sequence. 2Since leukemias important and complementary uses: and lymphomas originate from the malignant transformation ofReagents - Ion S5/PGM Detection1.Detection of clonal populations. individual lymphoid cells, all leukemias and lymphomas generally share one or more cell-specific or clonal antigen receptor geneAssay Components2.dentification of sequence information and gene rearrangements. Therefore, tests that detect IGK clonal populations I Master Mix Name Index # Master Mix Name Index #segment utilization. can be useful in the study of B-cell malignancies and complement IGH 3. Ability to track sequences in subsequent samples with the testing, as the IGK receptor is less susceptible to somatic mutations. IGK S5/PGM 01 IonXpress_001 IGK S5/PGM 11 IonXpress_011 use of the LymphoTrack MRD Software. IGK S5/PGM 02 IonXpress_002 IGK S5/PGM 12 IonXpress_012Unlike conventional fragment analysis assays, this NGS methodSpecimen Requirement IGK S5/PGM 04 IonXpress_004 IGK S5/PGM 13 IonXpress_013utilizes a single multiplex master mix to target conserved regions50 ng of high-quality genomic DNA. IGK S5/PGM 08 IonXpress_008 IGK S5/PGM 14 IonXpress_014of IGK that are described in lymphoid malignancies. Primers are designed with Illumina adapters and indices (8-24) or ThermoIGK S5/PGM 09 IonXpress_009 IGK S5/PGM 16 IonXpress_016Fisher Scientific adaptors and indices (12), thereby allowing for IGK S5/PGM 010 IonXpress_010 IGK S5/PGM 17 IonXpress_017a one-step PCR reaction to generate sequence-ready amplicons. ReferencesTo reduce per sample testing costs, amplicons from different1.S Tonegawa et al., Nature 302: 575-581 (1983). Controlssamples (amplified with different indexed master mixes) or2. JE Miller et al., Molecular Genetic Pathology (2nd ed.). SpringerIGK POS (+) Qty. 2 NGS NEG (-) Qty. 2LymphoTrack kits can be sequenced together on a single IlluminaMiSeq flow cell, Ion S5 or PGM chips. Science & Business Media. 2013: 30.2.7.13 and 30.2.7.18. V-J Sequence Frequencies. The LymphoTrack bioinformatics software provides a stacked bar graph depicting the relative frequencies for the 200 most prevalent rearrangements sequenced and identified in the sample. To learn more about the LymphoTrack software, please refer to the The LymphoTrack bioinformatics software enables simplifiedLymphoTrack Bioinformatics Software section.analysis and visualization of data and the LymphoTrack MRD Software allows identified sequences to be tracked in subsequent samples. Please see the LymphoTrack MRD software section to learn how the LymphoTrack Assays can be applied to MRD studies, or email marketing@invivoscribe.com. Positive (clonal) and negative (polyclonal) DNA controls are included in the kits.Ordering InformationCatalog # Products Quantity ComponentsSimplified Representation of the IGK Gene 7-122-0009 LymphoTrack IGK Assay Kit A - MiSeq8 indices - 5 sequencing reactions each7-122-0019 LymphoTrack IGK Assay Panel - MiSeq24 indices - 5 sequencing reactions eachDepicted are the variable region (V) genes or variable7-500-0009 LymphoTrack Software - MiSeq1 CD complimentary with purchaseintragenic J-C intron (J-C INTR) and downstream consensus joining region genes (J) or kappa deleting7-122-0007 LymphoTrack IGK Assay - S5/PGM12 indices - 5 sequencing reactions eachKdeelement (K de ) that are involved in IGK gene rearrangements.7-500-0007 LymphoTrack Software - S5/PGM1 CD complimentary with purchaseKde7-500-0008 LymphoTrack MRD Software* 1 CD complimentary with purchase46*MRD Software can be used to track sequences generated by either LymphoTrack Assays - MiSeq or Ion S5/PGM. Invivoscribe 2021|47'