b'Next-Generation Sequencing (NGS) | Research Use Only (RUO) AssaysTRG Assay Next-Generation Sequencing (NGS) | RUO Assays TRG Assay Next-Generation Sequencing (NGS) | RUO AssaysReagents - MiSeq DetectionLymphoTrack TRG Assay Kit A Components Panel Components(includes all master mixes from Kit A plus the items below)Master Mix Name Index # Master Mix Name Index # Master Mix Name Index #TRG MiSeq 01 A001 TRG MiSeq 09 A009 TRG MiSeq 18 A018TRG MiSeq 02 A002 TRG MiSeq 10 A010 TRG MiSeq 19 A019Assay Uses BackgroundTRG MiSeq 03 A003 TRG MiSeq 11 A011 TRG MiSeq 20 A020This research use only (RUO) assay for next-generation sequencingThe human T-cell receptor gamma (TRG) gene locus on chromosomeTRG MiSeq 04 A004 TRG MiSeq 12 A012 TRG MiSeq 21 A021(NGS) identifies clonal TRG V-J rearrangements and the associated7 (7q14) includes 14 variable region (Group I, II, III, and IV), 5 joiningTRG MiSeq 05 A005 TRG MiSeq 13 A013 TRG MiSeq 22 A022V-J region DNA sequences. It also provides the frequency distributionregion (J) gene segments, and 2 constant (C) genes spread over TRG MiSeq 06 A006 TRG MiSeq 14 A014 TRG MiSeq 23 A023of V-J segment utilization using the Illumina MiSeq or Thermo Fisher200 kilobases. Scientific Ion S5 and PGM instruments. TRG MiSeq 07 A007 TRG MiSeq 15 A015 TRG MiSeq 25 A025During development of lymphoid cells, the antigen receptor genesTRG MiSeq 08 A008 TRG MiSeq 16 A016 TRG MiSeq 27 A027Summary and Explanation of the Test(including gene segments within the TRG locus), undergo somaticControls Controlsgene rearrangement. 1These developmentally regulated gene The LymphoTrack TRG Assay represents a significant improvementrearrangements generate V-J combinations that are unique forTRG POS (+) Qty. 1 NGS NEG (-) Qty. 1 TRG POS (+) Qty. 3 NGS NEG (-) Qty. 3over existing clonality assays that utilize fragment analysis byeach cell. 2Since leukemias and lymphomas originate from the providing three important and complementary uses: malignant transformation of individual lymphoid cells, all leukemias and lymphomas generally share one or more cell-specific or clonalReagents - Ion S5/PGM Detection1.Detection of clonal populations. antigen receptor gene rearrangements. Therefore, tests that detect 2.dentification of sequence information and gene TRG clonal populations can be useful in the study of T-cell and certainAssay ComponentsIsegment utilization. B-cell malignancies.Since the TRG locus rearranges before the TRBMaster Mix Name Index # Master Mix Name Index # Master Mix Name Index #3. Ability to track sequences in subsequent samples with the use oflocus, and all unsuccessful or unproductive rearrangements are TRG S5/PGM 01 IonXpress_001 TRG S5/PGM 07 IonXpress_007 TRG S5/PGM 11 IonXpress_011the Invivoscribe MRD Software. retained in the cells, examination of the TRG locus can identify both This assay utilizes a single multiplex master mix to target conservedclonal / as well as clonal / T-cells. Clonal / T-cells would beTRG S5/PGM 02 IonXpress_002 TRG S5/PGM 08 IonXpress_008 TRG S5/PGM 12 IonXpress_012V and J regions of the human TRB gene locus described in lymphoidexpected to have biallelic TRG gene rearrangements.TRG S5/PGM 03 IonXpress_003 TRG S5/PGM 09 IonXpress_009 TRG S5/PGM 13 IonXpress_013malignancies, thereby reducing sample DNA requirements andTRG S5/PGM 04 IonXpress_004 TRG S5/PGM 10 IonXpress_010 TRG S5/PGM 14 IonXpress_014simplifying the testing workflow. Primers are designed with Illumina Note: For a more thorough explanation of the locus and the targeted deep sequencing strategy, please refer to Principle of ImmunoglobulinControlsadapters and indices (8-24) or Thermo Fisher Scientific adaptorsand T-Cell Receptor Gene Rearrangement. 2 TRG POS (+) Qty. 2 NGS NEG (-) Qty. 2and indices (12), thereby allowing for a one-step PCR reaction to generate sequence-ready amplicons. In addition, amplicons from different samples (amplified with different indexed master mixes) orSpecimen Requirement V - J Sequence Frequencies. The LymphoTrack bioinformatics software provides a stacked bar graph depicting the relative frequencies for the V-J rearrangements LymphoTrack kits can be sequenced together on a single Illumina identified and sequenced in a sample. To learn more about the LymphoTrack software, MiSeq flow cell Ion S5 or PGM chip to reduce per sample testing50 ng of high-quality genomic DNA. please refer to the LymphoTrack Bioinformatics Software section.costs.The LymphoTrack bioinformatics software enables simplifiedReferencesanalysis and visualization of data generated from this assay and1.Tonegawa, S. (1983). Nature 302, 575-581.the LymphoTrack MRD Software allows sequences to be tracked in2. Miller JE. (2013) Molecular Genetic Pathologysubsequent samples. Please see the LymphoTrack MRD software(2nd Edition.,sections 30.2.7.13 and 30.2.7.18).section to learn how the LymphoTrack Assays can be applied to MRD studies, or email marketing@invivoscribe.com.Positive (clonal) and negative (polyclonal) DNA controls are included in the kits.Ordering InformationCatalog # Products Quantity Components7-227-0019 LymphoTrack TRG Assay Kit A - MiSeq8 indices - 5 sequencing reactions each7-227-0009 LymphoTrack TRG Assay Panel - MiSeq24 indices - 5 sequencing reactions eachSimplified Representation of theTRG Gene 7-500-0009 LymphoTrack Software - MiSeq1 CD complimentary with purchase7-227-0007 LymphoTrack TRG Assay - S5/PGM12 indices - 5 sequencing reactions each7-500-0007 LymphoTrack Software - S5/PGM1 CD complimentary with purchase7-500-0008 LymphoTrack MRD Software* 1 CD complimentary with purchaseSimple representation of the organization of the T-cell receptor gamma gene on chromosome 7. Depicted are the variable region genes (V2V11) and*MRD Software can be used to track sequences generated by either LymphoTrack Assays - MiSeq or Ion S5/PGM. downstream joining region genes (J1/2JP1/2) that are involved in rearrangements in T-cell lymphomas.48RUO products are for Research Use Only; not intended for in vitro diagnostic use. Invivoscribe 2021|49'