b'Reference | Support21.28.Do I need to perform an adapter ligation prior to sequencing Product List by Catalog NumberCan I use a different library quantification method or kit?We recommend using the KAPA kit for MiSeq assays andmy products?either the 2100 Bioanalyzer or the LabChip GX for the Ion S5/ Performing an adapter ligation is not needed. The primers PGM assays. included in our LymphoTrack and LymphoTrack Dx master mixesCompanion Diagnostic Assays2-101-0170 Hypermutation Mix 1 v2.0Unlabeled already include the appropriate index barcodes and adapter2-101-0171 Hypermutation Mix 1 v2.06FAM 22.Will the LymphoTrack or LymphoTrack Dx analysis softwaresequences. After PCR amplification, you will be able to proceedCapillaryAnalysis 2-101-0180 Hypermutation Mix 2 v2.0Unlabeled2-101-0181 Hypermutation Mix 2 v2.06FAM work on my computer? with amplicon purification, amplicon quantification, library2-102-0010 IGK Tube AUnlabeledK-412-0291LeukoStrat CDx FLT3 Mutation Assay (CE-IVD) The software requires Microsoft Windows 7 (64-bit) and Excelpooling, and sequencing. K-412-0281LeukoStrat CDx FLT3 Software (CE-IVD)2-102-0011 IGK Tube A6FAM 2-102-0020 IGK Tube BUnlabeled 2007, 2010, or 2013 and will work with most desktop or laptopK-412-0331LeukoStrat CDx FLT3 Mutation Assay (JP)2-102-0021 IGK Tube B6FAM 29.If the LymphoTrackor LymphoTrackDx software generated anK-412-0341LeukoStrat CDx FLT3 Software (JP) PCs. For specific requirements please refer to the software2-103-0010 IGL TubeUnlabeled error, what information should I submit to Technical Support? K-412-0361LeukoStrat CDx FLT3 Mutation Assay (USA)2-103-0011 IGL Tube6FAM instructions for use.K-412-0371LeukoStrat CDx FLT3 Software (USA) Please submit the *.txt Log file that should have been createdK-412-0381LeukoStrat CDx FLT3 Mutation Assay (AUS)2-205-0010 TCRB Tube AUnlabeled 2-205-0011 TCRB Tube A6FAM & HEX by the software in the output folder, a screenshot of the sampleK-412-0391LeukoStrat CDx FLT3 Software (AUS)2-205-0020 TCRB Tube BUnlabeled 23.or LymphoTrack Dx bioinformaticsdirectory, and the Lot Number of the software CD you are using2-205-0021 TCRB Tube B6FAM Can I use the LymphoTracksoftware with a different assay? to support@invivoscribe.com. Research Use Only Assays2-205-0030 TCRB Tube CUnlabeled No, the software will only work with datasets obtained by ourCapillary & Gel Fragment Analysis 2-205-0031 TCRB Tube C6FAM & HEX 2-206-0010 TCRD TubeUnlabeled 30.Are controls provided with the kits? Can you purchase2-206-0011 TCRD Tube6FAM & HEX LymphoTrack and LymphoTrack Dx Assays.additional controls? How are they supplied? 1-100-0010IGH + IGK B-Cell Clonality AssayGel Detection2-207-0010 T-Cell Receptor Gamma Mix 1Unlabeled 1-100-0031IGH + IGK B-Cell Clonality AssayABI Fluorescence Detection2-207-0020 T-Cell Receptor Gamma Mix 2Unlabeled 24.What characters can I use when naming my samples andEach kit contains the necessary positive and negative controls1-100-0041IGH + IGK B-Cell Clonality Assay MegaKitABI Fluorescence Detection2-207-0021 T-Cell Receptor Gamma Mix 2HEXthe file pathways? What types of files are accepted by therequired to perform the assay; additional controls may also be1-101-0010IGH Gene Rearrangement AssayGel Detection2-207-0071 T-Cell Receptor Gamma Mix 16FAM LymphoTrack and LymphoTrack Dx Software - MiSeq? purchased separately. Single-tube DNA controls are provided1-101-0020IGH Gene Clonality AssayGel Detection2-207-0091 TCRG6FAM 1-101-0040IGH Gene Clonality Assay MegaKitGel Detection2-308-0010 BCL1/J HTubeUnlabeled Our software only recognizes file names and pathways thatas 100 L aliquots of 200 g/mL in 1/10 TE Buffer, 50 L aliquots1-101-0051IGH Gene Rearrangement AssayABI Fluorescence Detection2-309-0010 BCL2/J Ht(14;18) (Mbr) Mix 1bUnlabeled contain the following characters (A-Z, a-z, 0-9, . (dot), _of 50 g/mL in 1/10 TE Buffer, and 45 L aliquots of 15 g/mL in1-101-0061IGH Gene Clonality AssayABI Fluorescence Detection2-309-0020 BCL2/J Ht(14;18) (mcr) Mix 2bUnlabeled 1/10 TE Buffer. Single-tube RNA controls are provided as 100 L1-101-0071IGH Gene Rearrangement Assay MegaKitABI Fluorescence Detection2-309-0030 BCL2/J Ht(14;18) (Mbr) Mix 1aUnlabeled (underscore), - (hyphen)). In addition, spaces in the pathname1-101-0081IGH Gene Clonality Assay MegaKitABI Fluorescence Detection aliquots of 400 g/ml in RNAse free in glass distilled water. 1-102-0020IGK Gene Clonality AssayGel Detection2-309-0040 BCL2/J Ht(14;18) (mcr) Mix 2aUnlabeled for the data files or software (pathnames include file folders2-309-0050 BCL2/J HTube AUnlabeled and file names) should be avoided. If the software encounters1-102-0021IGK Gene Clonality AssayABI Fluorescence Detection2-309-0060 BCL2/J HTube BUnlabeled 31.What are the differences between dilution sets, sensitivity panels,1-102-0030IGK Gene Clonality Assay MegaKitGel DetectionHa character that is not listed above or extra spaces, an error2-309-0070 BCL2/JTube CUnlabeled and proficiency panels? 1-102-0031IGK Gene Clonality Assay MegaKitABI Fluorescence Detection2-310-0010 BCR/ABL t(9;22) Mix 1aUnlabeled message may be generated. Furthermore, the software is1-103-0010IGL Gene Clonality AssayGel Detection 2-310-0020 BCR/ABL t(9;22) Mix 2aUnlabeled only compatible with adaptor-trimmed fastq.gz files that are31a.RNA Dilution Sets1-103-0011IGL Gene Clonality AssayABI Fluorescence Detection2-310-0030 BCR/ABL t(9;22) Mix 3aUnlabeled generated by the MiSeq Reporter Software when the MiSeqBCR/ABL b3a2 (Cat# 4-085-0210), BCR/ABL b2a2 (Cat#1-103-0020IGL Gene Clonality Assay MegaKitGel Detection2-310-0040 BCR/ABL t(9;22) Mix 1bUnlabeled instrument is used. An example of the naming format that the4-085-0310), and BCR/ABL e1a2 (Cat# 4-085-0110). These1-103-0021IGL Gene Clonality Assay MegaKitABI Fluorescence Detection2-310-0041 BCR/ABL t(9;22) Mix 1bHEX 1-205-0010TCRB Gene Clonality AssayGel Detection MiSeq Reporter uses: SampleName_S1_L001_R1_001.fastq.gzsets contain six tubes: 100% negative control RNA and1-205-0011TCRB Gene Clonality AssayABI Fluorescence Detection2-310-0050 BCR/ABL t(9;22) Mix 2bUnlabeled 2-310-0051 BCR/ABL t(9;22) Mix 2bHEX and SampleName_S1_L001_R2_001.fastq.gz. volume to volume (v/v) dilutions (10- 1 , 10- 2 , 10- 3 , 10- 4 , and1-205-0020TCRB Gene Clonality Assay MegaKitGel Detection2-310-0060 BCR/ABL t(9;22) Mix 2cUnlabeled 10- 5 ) of the positive control RNA into the negative control1-205-0021TCRB Gene Clonality Assay MegaKitABI Fluorescence Detection2-310-0061 BCR/ABL t(9;22) Mix 2cHEX Do the Invivoscribe MiSeq 1-206-0010TCRD Gene Clonality AssayGel Detection 25.indices correspond to the RNA (IVS-0048). The RNA Dilution Sets are supplied at2-310-0070 BCR/ABL t(9;22) Mix 3bUnlabeled 1-206-0011TCRD Gene Clonality AssayABI Fluorescence Detection Illumina indices? a concentration of 400 g/mL, and each tube contains1-206-0020TCRD Gene Clonality Assay MegaKitGel Detection2-310-0071 BCR/ABL t(9;22) Mix 3b6FAM 2-310-0080 BCR/ABL t(9;22) Mix 3cUnlabeled The indices included in our MiSeq master mixes follow50 L. These dilution sets may be used to establish a1-206-0021TCRD Gene Clonality Assay MegaKitABI Fluorescence Detection2-310-0081 BCR/ABL t(9;22) Mix 3c6FAMstandard reference curve, as proficiency controls, as1-207-0010T-Cell Receptor Gamma Gene Rearrangement AssayGel Detection2-310-0090 BCR/ABL t(9;22) Mix 3dUnlabeled Illumina s TruSeq LT nomenclature. For instance, IGH FR11-207-0051T-Cell Receptor Gamma Gene Rearrangement AssayABI Fluorescence Detection MiSeq 01 corresponds to A001. Information for the other indicessensitivity controls for specific target assays, and as1-207-0101T-Cell Receptor Gamma Gene Rearrangement Assay 2.02-310-0101 BCR/ABL t(9;22) Mix 3d6FAM routine testing controls for cDNA synthesis, amplificationABI Fluorescence Detection2-311-0011 PML/RARa t(15;17) Mix 1HEX can be found in the instructions for use on how to set up the2-311-0031 PML/RARa t(15;17) Mix 2bHEX MiSeq Sample Sheet to detect the appropriate indices. and detection. 1-207-0111T-Cell Receptor Gamma Gene Rearrangement Assay 2.0 MegaKit2-311-0041 PML/RARa t(15;17) Mix 2cHEXABI Fluorescence Detection 1-308-0010BCL1/JTranslocation AssayGel Detection 26.Why am I getting a low percent passing filter and Q30 score? 31b.RNA Sensitivity Panels 1-309-0010Ht(14;18) Translocation AssayGel DetectionBCR/ABL RNA Dilution SetsBCL2/J HThese panels consist of seven tubes: 100% positive control1-310-0010BCR/ABL t(9;22) Translocation AssayGel Detection Low Q30 and percent passing filter (%PF) scores couldRNA and v/v dilutions (10- 1 , 10- 2 , 10- 3 , 10- 4 , 10- 5 , and 10- 6 )1-310-0031BCR/ABL t(9;22) Translocation AssayABI Fluorescence Detection4-085-0110 BCR/ABL e1a2 RNA Dilution Set be an indication that the flow cell is overloaded. If this isof the positive control RNA into the negative control RNA1-311-0010PML/RARa t(15;17) Translocation AssayGel Detection4-085-0210 BCR/ABL b3a2 RNA Dilution Set suspected, verify your amplicon and library calculations(IVS-0035). The RNA Sensitivity Panels are supplied at a1-412-0010FLT3 Mutation Assay - Gel Detection 4-085-0310 BCR/ABL b2a2 RNA Dilution Setand quantifications are correct. Low run metrics can also be1-412-0020FLT3 Mutation Assay MegaKit - Gel Detection concentration of 400 g/mL, and each tube contains 1001-412-0031FLT3 Mutation Assay - ABI Fluorescence Detectionattributed to many additional factors including poor qualityL. The RNA Sensitivity Panels may be used as sensitivity1-412-0041FLT3 Mutation Assay MegaKit - ABI Fluorescence DetectionDNA Sensitivity PanelsDNA, contamination, flow cell or instrument issues, etc. Pleasecontrols for specific target assays, and as routine testing4-086-0040 Sensitivity PanelIVS-0004 Clonal Control DNA refer to your Illumina MiSeq user guides and contact Illuminacontrols for cDNA synthesis, amplification and detection. Master Mixes 4-086-0070 Sensitivity PanelIVS-0007 Clonal Control DNA Support. 9-227-0007 LymphoTrack Dx TRG AssayS5/PGM 31c. DNA Sensitivity Panels 2-096-0010 Amplification Control Master MixUnlabeled9-500-0009 LymphoTrack Dx SoftwareMiSeq 2-096-0011 Amplification Control Master Mix6FAM9-121-0099 LymphoTrack Dx IGH FR2 Assay PanelMiSeq 27.Why is the same V H -J Hrearrangement combination andConsist of six tubes: 100% clonal DNA and v/v dilutions of the 2-096-0020Specimen Control Size LadderUnlabeled9-121-0109 LymphoTrackDx IGH FR3 Assay Kit AMiSeqsequence shared by two groups of reads, one of which is severalclonal DNA into negative polyclonal DNA (IVS-0000) to make9-121-0119 LymphoTrack Dx IGH FR3 Assay PanelMiSeq 2-096-0021Specimen Control Size Ladder6FAM bases shorter than the other when looking at the Read Summary30%, 20%, 10%, 5%, and 1% dilutions. The DNA Sensitivity Panels2-101-0010IGH Tube AUnlabeled9-121-0057 LymphoTrack Dx IGH FR1/2/3 AssayPGM tab of the excel document created by the LymphoTrackare supplied at a concentration of 200 g/mL and each tube2-101-0011IGH Tube A6FAM9-121-0007 LymphoTrack Dx IGH FR1 AssayPGM 2-101-0020IGH Tube BUnlabeled9-121-0037 LymphoTrack Dx IGH FR2 AssayPGM Visualization Tool? contains 100 L. The DNA Sensitivity Panels may be used as 2-101-0030 IGH Tube CUnlabeled9-121-0047 LymphoTrackDx IGH FR3 AssayPGMOur software was designed to list every unique sequencesensitivity controls for specific target assays. 2-101-0031 IGH Tube CHEX9-122-0009 LymphoTrack Dx IGK Assay Kit AMiSeq 2-101-0040 IGH Tube DUnlabeled9-122-0019 LymphoTrack Dx IGK Assay PanelMiSeq separately in order for the customer to see all of the data and31d.RNA Proficiency Panel2-101-0041 IGH Tube DHEX9-122-0007 LymphoTrackDx IGK AssayS5/PGMmake their own determination on how to interpret it. The several9-225-0009 LymphoTrack Dx TRB Assay Kit A - MiSeq The proficiency panel for BCR-ABL1 t(9;22) can be used2-101-0050 IGH Tube EUnlabeled base pair difference can be due to a number of factors including2-101-0051 IGH Tube E6FAM9-225-0019 LymphoTrackDx TRB Assay Panel - MiSeqamplification errors and sequencing errors. It could also be aas a sensitivity control for specific target assays, and as2-101-0060 IGH Framework 1 (FR1)Unlabeled9-227-0019 LymphoTrack Dx TRG Assay Kit AMiSeq routine testing controls for cDNA synthesis, amplification2-101-0061 IGH Framework 1 (FR1)6FAM9-227-0009 LymphoTrack Dx TRG Assay PanelMiSeq result of similarities between some of the primer sequences thatand detection. It consists of ten tubes: 100% positive control2-101-0070 IGH Framework 2 (FR2)Unlabeled9-500-0007 LymphoTrack Dx SoftwareS5/PGM were designed to ensure maximum coverage. We also include aRNA and v/v dilutions (10- 2and 10- 4 ) of IVS-0003, IVS-00112-101-0081 IGH Framework 3 (FR3)HEX Merged Read Summary report for your reference that combines2-101-0091 IGH Framework 2 (FR2)6FAMNext-Generation Sequencingsequences that only differ by 1 or 2 basepairs. and IVS-0032. It also includes BCR-ABL1 Negative Clonal2-101-0101 IGH Tube B6FAM Control RNA (IVS-0035).156Invivoscribe 2021|157'