b'Gel & Capillary | RUO AssaysGel and Capillary Gel and CapillaryBCL1/J HTranslocation AssayResearch Use Only (RUO) Assay BCL1/JH Translocation Assay Research Use Only (RUO) AssayBCL1/JTranslocation AssayHAssay Use Specimen RequirementsThese assays identify BCL1/J Ht(11;14) translocations and are useful forThis assay tests extracted and purified genomic DNA (gDNA).the evaluation of new research and methods in malignancy studies. ReagentsSummary and Explanation of the TestReferences Controls Concentration Units in Assay Units in Assay MegaKitTwo master mixes are included in this assay kit. The BCL1/J HMaster1.P Wijers et al., Leukemia 17: 2296-2298 (2003). IVS-0010 Clonal Control DNA 200 g/mL 1 x 100 L tube 5 x 100 L tubesMix targets the major translocation cluster (MTC) of the CCND12. JJM van Dongen et al., Leukemia 17: 2257-2317 (2003).locus (formerly known as BCL1) and the joining region (J H ) of the3. Shimazaki C, et. al., (1997).International Journal of Hematology.IVS-0000 Polyclonal Control DNA 200 g/mL 1 x 100 L tube 5 x 100 L tubesimmunoglobulin heavy chain locus (IGH). The Specimen Control Size66(1):111-5. Master Mixes Target Units in Assay Units in Assay MegaKitLadder Master Mix targets multiple genes and generates a series ofBCL1/J HTube MTC of CCND1 + IGH J H 1 x 1500 L tube 10 x 1500 L tubesamplicons of approximately 100, 200, 300, 400, and 600 base pairs to ensure that the quality and quantity of input DNA is adequate toSpecimen Control Size Ladder Multiple Genes 1 x 1500 L tube 10 x 1500 L tubesyield a valid result. Positive and negative controls are included. PCR products can be analyzed using standard gel electrophoresis with ethidium bromide staining. A CCND1 translocation is indicated if the master mix generates product(s) within the valid size range.Ordering InformationBackgroundCatalog # Products QuantityThis aberrant BCL1/J Ht(11;14) translocation juxtaposes genes of the1-308-0010 BCL1/J HTranslocation Assay - Gel Detection 33 reactionsimmunoglobulin heavy chain (IGH) gene on chromosome 14q32 with the cyclin D1 gene on chromosome 11q13.The juxtaposition of IGH-sequences results in the transcriptional activation of cyclin D1. 2,3 Cyclin D1 is involved in the regulation of the G1 progression and G1/S transition of the cell cycle. 3Translocation does not lead to expression of a fusion protein. In fact, oncogenesis is due to a promoter/enhancer exchange, wherein the immunoglobulin gene enhancer stimulates the expression of cyclin D1. Overexpression of cyclin D1, in turn, accelerates passage of transformed cells through the G1 phase.This assay is based on the EuroClonality/BIOMED-2 Concerted Action BMH4-CT98-3936.Figure Legend: Schematic diagram of the IGH-CCND1 t(11;14) translocation showing the cyclin D1 (CCND1) gene on the left and the Ig heavy chain (IGH) gene on the right. Shown are the relative positions and orientations for the BCL1/MTC primer and the JH primer, which are included in the BCL1/J HMaster Mix tube.114Invivoscribe 2021|115'