b'Gel and CapillaryIGH Gene Rearrangement Assays Research Use Only (RUO) AssayReagentsControls Concentration Units in Assay Units in Assay MegaKitIVS-0030 Clonal Control DNA 200 g/mL 1 x 100 L tube 5 x 100 L tubesIVS-0029 Clonal Control DNA 200 g/mL 1 x 100 L tube 5 x 100 L tubesIVS-0000 Polyclonal Control DNA 200 g/mL 1 x 100 L tube 5 x 100 L tubesMaster Mixes Target Units in Assay Units in Assay MegaKitIGH Framework 1 Framework 1 + J H 1 x 1500 L tube 10 x 1500 L tubesIGH Framework 2 Framework 2 + J H 1 x 1500 L tube 10 x 1500 L tubesIGH Framework 3 Framework 3 + J H 1 x 1500 L tube 10 x 1500 L tubesAmplification Control HLA-DQa 1 x 1500 L tube 10 x 1500 L tubesGel Electrophoresis Detection The data shown was generated using the master mix indicated. Amplified products were run on a 6% non-denaturing polyacrylamide/TBE gel. Lane 1 is data generated testing an alternative 100% clonal control DNA; lane 2 is data generated testing the recommended 100% clonal control DNA; lane 3 is data generated testing a 10% dilution of the recommended clonal control DNA; and, lane 4 is data generated testing IVS-0000 Polyclonal Control DNA (Cat# 4-092-0010). IVS-0000 is used as a negative control for many of our tests. A standard 100 base pair DNA size ladder was run in the lanes flanking the test samples.Capillary Electrophoresis Detection (ABI)The data shown was generated using the master mix indicated. Amplified products were run on an ABI 3100 instrument. Panel 1 displays data generated testing an alternative 100% clonal control DNA; panel 2 displays data generated testing the recommended 100% clonal control DNA; panel 3 displays data generated testing a 10% dilution of the recommended clonal control DNA; and, panel 4 displays data generated testing the IVS-0000 Polyclonal Control DNA (Cat# 4-092-0010). Our IVS-0000 DNA is often tested to provide information regarding valid size ranges for each master mix.Ordering InformationCatalog # Products Quantity1-101-0010IGH Gene Rearrangement Assay - Gel Detection30 reactions1-101-0030 IGH Gene Rearrangement Assay MegaKit - Gel Detection 300 reactions1-101-0051IGH Gene Rearrangement Assay - ABI Fluorescence Detection30 reactions1-101-0071 IGH Gene Rearrangement Assay MegaKit - ABI Fluorescence Detection300 reactionsInvivoscribe 2020|99'