b'Gel and CapillaryPML/RARa t(9;22) Translocation Assays Research Use Only (RUO) AssayReagentsControls Concentration Units in Assay Units in Assay MegaKitIVS-0020 Clonal Control RNA 400 g/mL 1 x 100 L tube 5 x 100 L tubesIVS-0035 Clonal Control RNA 400 g/mL 1 x 100 L tube 5 x 100 L tubesMaster Mixes Target Units in Assay Units in Assay MegaKitPML/RAR t(15;17) Mix 1 RARA 1 x 1500 L tube 10 x 1500 L tubesPML/RAR t(15;17) Mix 2a PML-RARA 1 x 1500 L tube 10 x 1500 L tubesPML/RAR t(15;17) Mix 2b S- and L-Forms 1 x 1500 L tube 10 x 1500 L tubesPML/RAR t(15;17) Mix 2c L-Form 1 x 1500 L tube 10 x 1500 L tubesGel Electrophoresis Detection The data shown were generated using the master mix indicated. Amplified products were run on a 6% polyacrylamide/TBE gel. Lane 1 is data generated testing cDNA synthesized from the recommended 100% clonal control RNA; lane 2 is data generated testing cDNA synthesized froma 10 -4dilution of the recommended clonal control RNA; and, lane 3 is data generated testing cDNA synthesized from the negative control the negative control IVS-0035 Clonal Control RNA (Cat# 4-089-3070). A standard 100 base pair DNA size ladder was run in the lanes flankingthe test samples.Capillary Electrophoresis Detection (ABI)The data shown were generated on an ABI 3100 instrument using the master mix indicated. Panel 1 represents data generated testing cDNA synthesized from the recommended 100% clonal control RNA; panel 2 represents data generated testing cDNA synthesized from a 10 -4dilution of the recommended clonal control RNA; and, panel 3 represents data generated testing cDNA synthesized from the negative control RNA. The positive RNA was diluted into the negative control RNA, IVS-0035 (Cat# 4-089-3070).Ordering InformationCatalog # Products Quantity1-311-0010 PML/RAR t(15;17) Translocation Assay - Gel Detection 30 reactions1-311-0020 PML/RAR t(15;17) Translocation Assay MegaKit - Gel Detection 300 reactions1-311-0011PML/RAR t(15;17) Translocation Assay - ABI Fluorescence Detection 30 reactions1-311-0021PML/RAR t(15;17) Translocation Assay MegaKit - ABI Fluorescence Detection 300 reactionsInvivoscribe 2020|127'