b'Bioinformatics Software Next-Generation Sequencing (NGS) | CE-IVD AssaysSequencing SummaryUsing the merged read summary, along with the easy to follow flow charts in the accompanying LymphoTrack Dx Assay instructions for use (IFU), interpretation is quick and easy.Sample Name1Total reads = 32,4582 3 4 5Merge% TotalCumulative Mutation rate In-frameNo stop Rank Sequence Length count V-gene J-gene reads %partial V-gene(Y/N) codon V-coverage(%) (Y/N)1 TTCTCGTGGTG 455 29603 IGHV4-59_08 IGHJ4_02 9.93 9.93 11.26 Y Y 98.632 CTCGCCCTCCT 463 205 IGHV5-51_01 IGHJ4_02 0.07 9.99 0.00 Y Y 99.663 GGTTTTCCTTG 484 201 IGHV3-7_01 IGHJ4_02 0.07 10.06 7.77 Y Y 100.004 CTCGCCCTCCT 463 185 IGHV5-51_01 IGHJ5_02 0.06 10.12 6.08 Y Y 99.325 CTCGCCCTCCT 469 170 IGHV5-51_01 IGHJ4_02 0.06 10.18 0.00 Y Y 99.326 CTCGCCCTCCT 466 160 IGHV5-51_01 IGHJ4_02 0.05 10.23 0.00 Y Y 99.667 CTGCTGCTGAC 460 159 IGHV2-5_10 IGHJ5_02 0.05 10.29 8.08 Y Y 97.648 GGTTTTCCTTG 493 156 IGHV3-48_02 IGHJ6_02 0.05 10.34 3.72 Y Y 98.999 CTCGCCCTCCT 334 153 IGHV5-51_02 IGHJ2_01 0.05 10.39 3.72 Y N 27.7010 CTCGCCCTCCT 334 152 IGHV5-51_02 IGHJ2_01 0.05 10.44 3.38 Y N 26.0111. The sample name is clearly identified and the total number of reads (= Read Depth) generated for the sample is provided. Following the IFU, it is easy to determine whether the data generated for a sample can be assessed for the presence or absence of clonality.2.The sequence of clonal populations is provided and populations are ranked from most abundant to least prevalent. Sequences 2that differ by 1-2 basepairs are automatically merged to account for possible sequencing errors and to improve the accuracy and ease of sample interpretation.3.Sequences are aligned with reference genes to allow for easy identification of specific types of gene rearrangements such as IGHV3-21,3which is characteristic of some CLL cases and correlates with a poor prognosis.4.The percentage that a unique sequence contributes to the total number of reads for a sample is calculated. Following the guidelines in the 4IFU, samples can be interpreted for the evidence indicating the presence or absence of clonality.5. For the LymphoTrack Dx IGHV Leader and IGH FR1 Assays, the somatic hypermutation status of a sequence is automatically calculated by 5comparing the identified sequence with a germline reference. In addition, predictions on whether the sequence would be functional can be drawn by the provided information regarding the presence of a premature stop codon or an open reading frame that is out-of-frame.Ordering InformationCatalog # Products Quantity Components9-500-0009 LymphoTrack Dx Software - MiSeq 1 CD complimentary with kit purchase9-500-0007 LymphoTrackDx Software - S5/PGM 1 CD complimentary with kit purchaseThese products are CE-IVD assays for in vitro diagnostic use.Invivoscribe 2020|33'