b'Gel & Capillary | RUO AssaysGel and CapillaryBCL1/JH Translocation AssayResearch Use Only (RUO) AssayBCL1/JH Translocation AssayAssay UseThe BCL1/J HTranslocation Assay is useful for studies involving: multiple myeloma (20-25%). The t(11;14) brings about juxtaposition of the cyclin D1 gene with the immunoglobulin heavy chain gene. Thisdentification of IGH-BCL1 (now known as IGH-CCND1) gene I leads to a marked increase in expression of cyclin D1 driven by the Ig rearrangements highly suggestive of mantle cell lymphoma heavy chain gene enhancer, located in the intron between the J Hand Lineage determination of leukemias and lymphomas constant region genes. The overexpression of cyclin D1 accelerates the Monitoring and evaluation of disease recurrence passage of transformed cells through the G1 phase. Approximately 41% of the breakpoints on the CCND1/MTC locus can be detected by PCR Detection and assessment of residual disease methodology. However, breakpoints outside of the CCND1/MTC locus Evaluation of new research and methods in malignancy studies will not be identified by this particular test. Therefore, a negative result does not completely exclude the presence of an IGH-CCND1 gene translocation in the sample. 1 Summary and Explanation of the TestTwo master mixes are included in this assay kit. The BCL1/J HMasterSpecimen RequirementsMix targets the major translocation cluster (MTC) of the CCND1 locus (formerly known as BCL1) and the joining region (J H ) of the5 mL of peripheral blood, bone marrow biopsy, or bone marrow immunoglobulin heavy chain locus (IGH). The Specimen Control Size Ladder Master Mix targets multiple genes and generates a series ofaspirate anti-coagulated with heparin or EDTA; or,amplicons of approximately 100, 200, 300, 400, and 600 base pairsMinimum 5 mm cube of tissue; or,to ensure that the quality and quantity of input DNA is adequate to2 g of genomic DNA; or,yield a valid result. Positive and negative controls are included. PCR products can be analyzed using standard gel electrophoresis withFormalin-fixed, paraffin-embedded (FFPE) tissue or slides.ethidium bromide staining. A CCND1 translocation is indicated if the master mix generates product(s) within the valid size range. ReferenceBackground1.P Wijers et al., Leukemia 17: 2296-2298 (2003).2. JJM van Dongen et al., Leukemia 17: 2257-2317 (2003).The IGH-CCND1 t(11;14)(q13;q32) translocation is mainly found in mantle cell lymphomas, but has also been seen in B-prolymphocytic leukemia (10-20%), plasma cell leukemia, splenic lymphoma with villous lymphocytes, chronic lymphocytic leukemia (2-5%), and in This assay is based on the EuroClonality/BIOMED-2 Concerted Action BMH4-CT98-3936.Figure Legend: Schematic diagram of the IGH-CCND1 t(11;14) translocation showing the cyclin D1 (CCND1) gene on the left and the Ig heavy chain (IGH) gene on the right. Shown are the relative positions and orientations for the BCL1/MTC primer and the JH primer, which are included in the BCL1/J HMaster Mix tube.118'