b'Gel & Capillary | RUO AssaysGel and CapillaryTCRB + TCRG T-Cell Clonality AssaysResearch Use Only (RUO) AssayTCRB + TCRG T-Cell Clonality AssaysAssay UseTCRB + TCRG T-Cell Clonality Assays are useful for studies involving: The T-cell receptor gamma (TRG, formerly known as TCRG) chain locus spans 128 kb on chromosome 7 (7p14). Rearrangement of the Identification of clonal T-cell populations highly suggestivevariable (V ) and joining (J ) genetic segments of the TRG locus result of T-cell malignancies in V-J products of unique length and sequence. The TRG locus does Lineage determination of leukemias and lymphomas not contain D segments. 2In addition, the TRG gene contains a limited Monitoring and evaluation of disease recurrence number of V and J segments such that the amplification of all major V-J combinations is possible with four V and two J primers.Detection and assessment of residual disease This standardized multiplex PCR assay detects the vast majority of Evaluation of new research and methods in malignancy studies clonal TRG gene rearrangements using two multiplex master mixes. 3 Using this assay, the clonal TRG gene rearrangement detectionrate is exceptionally high. 3Summary and Explanation of the Test PCR products generated from the TRB and TRG assays are easily Five master mixes are included in these test kits to test forand reliably identified by heteroduplex analysis or capillary rearrangements of both TRB (formerly known as TCRB) and TRGelectrophoresis.(formerly known as TCRG). TCRB Tubes A and B target framework regions within the variable region, and the joining region of theT-cell receptor beta locus. TCRB Tube C targets the diversity andSpecimen Requirementsjoining regions. TCRG Tubes A and B target framework regions within the variable region, and the joining region of the T-cell receptor5 mL of peripheral blood, bone marrow biopsy, or bone marrow gamma locus.aspirate anti-coagulated with heparin or EDTA; or,Positive and negative controls, as well as a Specimen Control SizeMinimum 5 mm cube of tissue; or,Ladder Master Mix, are included. PCR products can be analyzed3 g of genomic DNA; or,by capillary electrophoresis or heteroduplex analysis. Clonality is indicated if any one of the master mixes generates clonal products. Formalin-fixed, paraffin-embedded (FFPE) tissue or slides.ReferenceBackground1.M Bruggemann et al., Leukemia 17: 2283-2289 (2003).The human T-cell receptor beta (TRB, formerly known as TCRB)2. JJM van Dongen et al., Leukemia 17: 2257-2317 (2003).gene locus on chromosome 7 (7q34, formerly 7q35) includes 64-673. K Beldjord et al., Leukemia 17: 2289-2292 (2003).variable (V) gene segments (belonging to 30 subgroups), 2 diversity (D) gene segments, and 13 joining (J) gene segments, spread over 685 kilobases. The diversity of this locus has complicated PCR-based testing, however, this standardized multiplex PCR assay detects the vast majority of clonal TRB gene rearrangements using only three multiplex master mixes. The detection rate of clonal TRB gene rearrangements using this assay is exceptionally high. 1 This assay is based on the EuroClonality/BIOMED-2 Concerted Action BMH4-CT98-3936.Figure Legend: Simplified diagram of a representative rearranged T-cell receptor beta gene and the T-cell receptor gamma gene showing the approximate placement of the upstream and downstream DNA primers.The numbers of primers and their specificity are listed for master mix TCRB Tubes A, B, and C and TCRG Tubes A and B. (The V1f primer is a consensus primer that targets V 1 through V 8).106'