b'Gel and CapillaryBCR/ABL t(9;22) Translocation AssaysResearch Use Only (RUO) AssayReagentsControls Concentration Units in Assay Units in Assay MegaKitIVS-0032 Clonal Control RNA 400 g/mL 1 x 100 L tube 5 x 100 L tubesIVS-0011 Clonal Control RNA 400 g/mL 1 x 100 L tube 5 x 100 L tubesIVS-0035 Clonal Control RNA 400 g/mL 1 x 100 L tube 5 x 100 L tubesMaster Mixes Target Units in Assay Units in Assay MegaKitBCR/ABL t(9;22) Mix 1a Abl1 x 1500 L tube 10 x 1500 L tubesBCR/ABL t(9;22) Mix 2a p190 1 x 1500 L tube 10 x 1500 L tubesBCR/ABL t(9;22) Mix 3ap210+230 1 x 1500 L tube 10 x 1500 L tubesBCR/ABL t(9;22) Mix 1b Abl 1 x 1500 L tube 10 x 1500 L tubesBCR/ABL t(9;22) Mix 2b p190 1 x 1500 L tube 10 x 1500 L tubesBCR/ABL t(9;22) Mix 2c p190 1 x 1500 L tube 10 x 1500 L tubesBCR/ABL t(9;22) Mix 3b p210+230 1 x 1500 L tube 10 x 1500 L tubesBCR/ABL t(9;22) Mix 3c p210+230 1 x 1500 L tube 10 x 1500 L tubesBCR/ABL t(9;22) Mix 3d p210+230 1 x 1500 L tube 10 x 1500 L tubesGel Electrophoresis Detection The data shown was generated using the master mix indicated. Amplified products were run on a 6% polyacrylamide/TBE gel. Lane 1 is data generated testing cDNA synthesized from an alternative 100% clonal control RNA; lane 2 is data generated testing cDNA synthesized from the recommended 100% clonal control RNA; lane 3 is data generated testing cDNA synthesized from a 10 -4dilution of the recommended clonal control RNA. The positive RNA was diluted into the negative control RNA, IVS-0035. A standard 100 base pair DNA size ladder was run in the lanes flanking the test samples.Capillary Electrophoresis Detection (ABI)The data shown was generated on an ABI 3100 instrument using the master mix indicated. Panel 1 represents data generated testing cDNA synthesized from an alternative 100% control RNA; panel 2 represents data generated testing cDNA synthesized from the recommended 100% clonal control RNA; and, panel 3 represents data generated testing cDNA synthesized from a 10 -4dilution of the recommended clonal control RNA. The positive RNA was diluted into the negative control RNA, IVS-0035 (Cat# 4-089-3070).Ordering InformationCatalog # Products Quantity1-310-0010 BCR/ABL t(9;22) Translocation Assay - Gel Detection 30 reactions1-310-0020 BCR/ABL t(9;22) Translocation Assay MegaKit - Gel Detection 300 reactions1-310-0031 BCR/ABL t(9;22) Translocation Assay - ABI Fluorescence Detection 30 reactions1-310-0041 BCR/ABL t(9;22) Translocation Assay MegaKit - ABI Fluorescence Detection 300 reactionsInvivoscribe 2020|125'