b'TCRD Gene Clonality Assays Gel and CapillaryAssaysReagentsControls Concentration Units in Assay Units in Assay MegaKitIVS-0021 Clonal Control DNA 200 g/mL 1 x 100 L tube 5 x 100 L tubesIVS-0000 Polyclonal Control DNA 200 g/mL 1 x 100 L tube 5 x 100 L tubesMaster Mixes Target Units in Assay Units in Assay MegaKitTCRD Tube Multiple V + D +J1 x 1500 L tube 10 x 1500 L tubesSpecimen Control Size Ladder Multiple Genes 1 x 1500 L tube 10 x 1500 L tubesGel Electrophoresis Detection Heteroduplex analysis is performed to differentiate clonal and non-clonal PCR products. It involves heat denaturation of double-stranded DNA, followed by a snap chilling process to force DNA strands to quickly reanneal. In non-clonal populations, this process causes a majority of the single-stranded DNA to incorrectly bind to non-homologous strands, resulting in secondary structures and reducing the DNAs ability to migrate through a non-denaturing polyacrylamide gel. As a result, polyclonal products are frequently observed as smears at high molecular weights. In clonal populations, after heteroduplex analysis is performed, most denatured single-strand PCR products will reanneal correctly with homologous strands of DNA (reforming homoduplexes), allowing them to easily migrate through the polyacrylamide gel as a single band. Therefore, heteroduplex analysis is key for analyzing PCR products visualized using gel detection methods, as it increases the separation between clonal and polyclonal products.Capillary Electrophoresis Detection (ABI)Differential fluorescence detection, such as ABI Fluorescence Detection, is commonly used to resolve different-sized amplicon products using a capillary electrophoresis instrument. Primers can be conjugated with several different fluorescent dyes (fluorophores), so that they produce different emission spectra upon excitation by a laser in the capillary electrophoresis instrument. In this manner, different fluorescent dyes can correspond to different targeted regions. This detection system results in excellent sensitivity, single nucleotide resolution, differential product detection, and relative quantification. In addition, differential detection allows accurate, reproducible and objective interpretation of primer-specific products. Inter-assay and intra-assay reproducibility in size determination using capillary electrophoresis is approximately 1 to 4 nucleotides.Ordering InformationCatalog # Products Quantity9-206-0010 IdentiClone TCRD Gene Clonality Assay - Gel Detection 33 reactions9-206-0020 IdentiClone TCRD Gene Clonality Assay MegaKit - Gel Detection 330 reactions9-206-0011 IdentiClone TCRD Gene Clonality Assay - ABI Fluorescence Detection33 reactions9-206-0021 IdentiClone TCRD Gene Clonality Assay MegaKit - ABI Fluorescence Detection 330 reactionsThese are in vitro diagnostic products, and are not available for sale or use within North America.Invivoscribe 2020|75'