b'Gel and CapillaryIGH Somatic Hypermutation Assays v2.0 Research Use Only (RUO) AssayReagentsControls Concentration Units in Assay Units in Assay MegaKitIVS-0013 Clonal Control DNA 200 g/mL 1 x 100 L tube 5 x 100 L tubesIVS-0013 Clonal Control RNA 400 g/mL 1 x 100 L tube 5 x 100 L tubesIVS-0000 Polyclonal Control DNA 200 g/mL 1 x 100 L tube 5 x 100 L tubesMaster Mixes Target Units in Assay Units in Assay MegaKitHypermutation Mix 1 v2.0 Leader + J H 1 x 1500 L tube 10 x 1500 L tubesHypermutation Mix 2 v2.0 Framework 1 + J H 1 x 1500 L tube 10 x 1500 L tubesSpecimen Control Size Ladder Multiple Genes 1 x 1500 L tube 10 x 1500 L tubesPrimers Target Units in Assay Units in Assay MegaKitPrimer - Hypermutation Leader + Framework 1 1 x 10 L tube at 100 M 5 x 10 L tubeIGH J HPrimer J H 1 x 10 L tube at 100 M 5 x 10 L tubeGel Electrophoresis Detection The data shown was generated using the master mix indicated. Amplified products were run on a 2% agarose/TBE gel. Lane 1 is data generated testing cDNA synthesized from the recommended 100% clonal control RNA; lane 2 is data generated testing the recommended 100% clonal control DNA; lane 3 is data generated testing a 10% dilution of the recommended clonal control DNA; and, lane 4 is data generated testing the IVS-0000 Polyclonal Control DNA (Cat# 4-092- 0010). Lanes flanking the samples were loaded with a 100 base pair DNA size standard.Capillary Electrophoresis Detection (ABI)The data shown was generated on an ABI 3130xl instrument using the master mix indicated. Panel 1 displays data generated testing cDNA synthesized from the recommended 100% clonal control RNA; panel 2 displays data generated testing the recommended 100% clonal control DNA; panel 3 displays data generated testing the recommended 10% clonal control DNA; and panel 4 displays data generated testing IVS-0000 Polyclonal Control DNA (Cat# 4-092-0010).Ordering InformationCatalog # Products Quantity5-101-0030 IGH Somatic Hypermutation Assay v2.0 - Gel Detection 33 reactions5-101-0040 IGH Somatic Hypermutation Assay v2.0 MegaKit - Gel Detection 330 reactions5-101-0031 IGH Somatic Hypermutation Assay v2.0 - ABI Fluorescence Detection 33 reactions5-101-0041 IGH Somatic Hypermutation Assay v2.0 MegaKit - ABI Fluorescence Detection 330 reactionsInvivoscribe 2020|129'