b'Controls | Control PanelsControls, Reagents, Controls, Reagents,and Enzymes and EnzymesDNA and RNA Sensitivity Panels Control PanelsDNA and RNA Sensitivity PanelsDNA Sensitivity Panels RNA Sensitivity PanelsDNA sensitivity panels are 6 member panels that consist of 100%RNA sensitivity panels are 7 member panels that consist of 100% clonal DNA extracted from a positive control cell line and 30%, 20%,clonal RNA extracted from a positive control cell line and 10 -1 , 10 -2 ,10%, 5%, and 1% dilutions of the positive clonal DNA diluted (v/v) into10 -3 , 10 -4 , 10 - 5 , and 10 -6(1:10 - 1:1 000 000) dilutions of the positive our standard negative control DNA, IVS-0000 Polyclonal Control DNA.clonal RNA diluted (v/v) into our standard negative control RNA,Each tube contains 100 L of DNA at a concentration of 200 g/mLIVS-0035 Clonal Control RNA. Each tube contains 100 L of RNA at in 1/10 TE buffer (1 mM Tris-HCl pH 8.0, 0.1 mM EDTA). This diluent400 g/mL in RNase-free glass-distilled water. The pH of distilled provides sufficient buffering capacity and EDTA to protect the DNA water is slightly acidic thereby protecting the RNA from hydrolysis. controls without interfering with the Mg 2+concentrations required Please note, these controls are for qualitative use only.for robust amplification reactions. Please note, these controls arefor qualitative use only.T Cell Receptor Gamma Mix 1 T Cell Receptor Gamma Mix 2100% IVS-0009Panel 1 100% IVS-0009Panel 1 30% IVS-0009Panel 230% IVS-0009Panel 2 20% IVS-0009Panel 3 20% IVS-0009Panel 310% IVS-0009Panel 4 10% IVS-0009Panel 45% IVS-0009Panel 5 5% IVS-0009Panel 51% IVS-0009Panel 6 1% IVS-0009Panel 6IVS-0000Panel 7 IVS-0000Panel 7This data was generated testing a Sensitivity Panel for IVS-0009 Clonal Control DNA using the master mixes listed. PCR products were run on an ABI 3130xL capillary electrophoresis instrument for differential fluorescence detection and data analyses. Panel 7 shows the polyclonal Gaussian distributions expected from our negative control IVS-0000 Polyclonal Control DNA. Data in the other panels are tests of positive control samples at the dilutions indicated. Clonal peaks (highlighted) are clearly evident in all of the positive sample panels.These products are research use only (RUO), not for diagnostic procedures. 146'