b'Gel & Capillary | RUO AssaysGel and CapillaryBCL2/JH Translocation AssayResearch Use Only (RUO) AssayBCL2/JH Translocation AssayAssay Use The t(14;18) brings about juxtaposition of BCL2 with the Ig heavy chain joining segment. This leads to a marked increase in expression The BCL2/J HTranslocation Assay is useful for studies involving: of BCL2 driven by the Ig heavy chain gene enhancer. The BCL2 Monitoring and evaluation of follicular lymphomas and otherprotein inhibits programmed cell death (apoptosis) leading to cell accumulation. The majority of breakpoints on 18q21-22 occur within B-cell lymphomas the major breakpoint region (MBR) in the 3 untranslated region of Distinguishing lymphoma from benign lymphoid hyperplasia exon 3 (60- 70% of the cases), and the minor cluster (mcr) region Distinguishing follicular lymphoma from other B-cell lymphomaslocated 3 to BCL2 exon 3 (20-25% of the cases). Some breakpoints occur at distant loci and will not be identified by this particular that may have a similar appearance test. Therefore, a negative result does not completely exclude the Monitoring and evaluation of disease recurrence presence of a BCL2/IGH gene rearrangement in the sample. 1In Detection and assessment of residual disease comparison, Southern blot analysis requires 1-2 weeks, is significantly less sensitive, and has more restrictive Specimen requirements.Evaluation of new research and methods in malignancy studies Summary and Explanation of the TestSpecimen RequirementsFour master mixes are included in this assay. Three are used to identify5 mL of peripheral blood, bone marrow biopsy, or bone marrowtranslocations in the major breakpoint region (MBR) and minor clusteraspirate anti-coagulated with heparin or EDTA; or,region (mcr) of BCL2. The Specimen Control Size Ladder masterMinimum 5 mm cube of tissue; or,mix targets multiple genes and generates a series of amplicons of approximately 100, 200, 300, 400, and 600 base pairs to ensure that2 g of genomic DNA; or,the quality and quantity of input DNA is adequate to yield a validFormalin-fixed, paraffin-embedded (FFPE) tissue or slides.result. This assay includes negative control DNA and positive control DNAs for both the MBR and mcr. PCR products can be analyzed using standard gel electrophoresis with ethidium bromide staining. A BCL2Referencetranslocation is indicated if any one of the master mixes generates1.PAS Evans et al., Leukemia 17: 2298-2301 (2003).product(s) within the valid size range.2. JJM van Dongen et al., Leukemia 17: 2257-2317 (2003).Background The BCL2 t(14;18)(q32;q21) translocation is found in 80-90% of follicular lymphomas and 30% of diffuse large cell lymphomas. The translocation is rarely present in other lymphoproliferative diseases. This assay is based on the EuroClonality/BIOMED-2 Concerted Action BMH4-CT98-3936.Figure Legend: Schematic diagram of the IGH-BCL2 t(14;18) translocation showing the BCL2 gene on the left and the Ig heavy chain (IGH) gene on the right. Shown are the relative positions and orientations for the major breakpoint region (MBR) primers, the minor cluster region (mcr) primers, and the J Hprimer, which are included in the 3 BCL2/J Hmaster mix tubes.122'