b'FLT3 Mutation Assay 2.0Gel and Capillary Assays andAssaysABI Fluorescence Detection ReagentsControls Concentration Units in Assay Units in MegaKitFLT3 ITD Positive Control 50 g/mL 1 x 100 L tube 5 x 100 L tubesFLT3 D835 Positive Control 50 g/mL 1 x 100 L tube 5 x 100 L tubesFLT3 Negative Control 50 g/mL 1 x 100 L tube 5 x 100 L tubesMaster Mixes Target Units in Assay Units in MegaKitFLT3 ITD Master Mix6FAM & HEX FLT3 ITD 1 x 1500 L tube 10 x 1500 L tubesFLT3 D835 Master Mix6FAM FLT3 TKD 1 x 1500 L tube 10 x 1500 L tubesCapillary Electrophoresis Detection (ABI)Differential fluorescence detection, such as ABI fluorescence detection, is commonly used to resolve different-sized amplicon products using a capillary electrophoresis instrument. Primers can be conjugated with different fluorescent dyes (fluorophores), so that they produce different emission spectra upon excitation by a laser in the capillary electrophoresis instrument. In this manner, different fluorescent dyes can correspond to different targeted regions. This detection system results in high sensitivity, single nucleotide resolution, differential product detection, and relative quantification. In addition, differential detection allows accurate, reproducible and objective interpretation of primer-specific products. Inter-assay and intra-assay reproducibility in size determination using capillary electrophoresis is approximately 1 to 4 nucleotides.The data shown was generated using the master mixes indicated. Amplified products were run on an ABI 3500xL instrument.Ordering InformationCatalog # Products Quantity9-412-0091 LeukoStrat FLT3 Mutation Assay 2.0ABI Fluorescence Detection 33 reactions9-412-0101 LeukoStrat FLT3 Mutation Assay 2.0 MegaKitABI Fluorescence Detection 330 reactionsThese are in vitro diagnostic products, and are not available for sale or use within North America.Invivoscribe 2020|85'