b'MRD TestsTest Name Next-generation sequencing of the PCR products is TRG MRD Clonality Assayused to identify DNA sequences specific to previously identified clonal rearrangements detected at diagnosis. Bioinformatics tools facilitate the detection of these Assay Type specific sequences present at MRD levels up to 1 x 10 -6with sufficient DNA input.Next-Generation Sequencing (NGS) For Research Use Only The assay typically requires a sample taken at diagnosis as well as the post-treatment follow-up samples. If the patient This test is performed by using the LymphoTrack Assayhas previously been tested by LabPMM for TRG clonality, from Invivoscribe. Data is analyzed using the LymphoTrackno diagnostic sample is needed.MRD Software (RUO).Method Description Indications for TestingIdentify tumor-specific markers for post-treatmentTo track and identify previously detected TRG clonalmonitoring sequences in post-treatment follow-up samples, aMonitor and evaluate disease recurrencemultiplex master mix targeting the V and the J regionis used for PCR amplification.Interpretation TurnaroundSpecimenShippingSpecimen Time Requirements Conditions StabilityAn interpretive5 to 14 1-3 mL of peripheral Ambient or Cool; 2-8 C up report will bebusiness daysblood in EDTA do not freezeto 7 days priorissued indicating0.25-1 mL of bone marrow (peripheral blood or to testingwhether TRG MRDin EDTA bone marrow) was detectedAmbient or frozen on 700-3500 ng of previously isolated DNA dependingdry ice (isolated DNA)on level of sensitivity requiredLabPMM Services Catalog 2021|47'