b'ClonalityTestsTest Name used to identify DNA sequences specific to clonal gene TRG Clonality Assayrearrangements. Bioinformatics tools facilitate the characterization of sequences present at greater than2.5% of the population. These sequences can beAssay Type used to track specific clonal populations.Next-Generation Sequencing (NGS) Indications for TestingThis test is performed by using the LymphoTrack ordentify clonality in atypical lymphoproliferative LymphoTrack Dx Assay from Invivoscribe. IdisordersSupport a differential diagnosis between reactiveMethod Description lesions and hematologic malignanciesFor detection of the vast majority of TRG geneAssign presumptive lineage in mature monoclonalrearrangements, a multiplex master mix targetinglymphoproliferative disordersthe V and J regions are used for PCR amplification.Monitor and evaluate disease recurrenceNext-generation sequencing of the PCR products is Interpretation TurnaroundSpecimenShippingSpecimen Time Requirements Conditions StabilityAn interpretive report will be5 to 10 1-3 mL of peripheralAmbient or2-8 C up issued indicating whetherbusiness daysblood in Heparin, Cool; doto 7 days prior evidence of clonality wasEDTA or ACD not freezeto testing detected. The report further0.25-1 mL of bone(peripheral provides a summary of theblood or bone top 5 merged sequences,marrow in Heparin,marrow) including the % total reads, theEDTA or ACD500 ng of previouslyAmbient or rearrangement class and the sequence. isolated DNAfrozen on dry ice (isolated DNA)LabPMM Services Catalog 2021|33'