b'ClonalityTestsTest Name framework region 3, as well as the joining region, is used IGH FR1 Clonality Assay for PCR amplification. Next-generation sequencing of the IGH FR2 Clonality AssayPCR products is used to identify DNA sequences specific to IGH FR3 Clonality Assayclonal gene rearrangements. Bioinformatics tools facilitate the characterization of sequences present at greater than 2.5% of the population. These sequences can be used to Assay Type track specific clonal populations.Next-Generation Sequencing (NGS) Indications for TestingThis test is performed by using the LymphoTrackdentify clonality in atypical lymphoproliferative Ior LymphoTrack Dx Assay from Invivoscribe disordersSupport a differential diagnosis between reactiveMethod Description lesions and hematologic malignanciesAssign presumptive lineage in mature monoclonal For detection of the vast majority of clonal IGH V H -J Hrearrangements, including the associated V H -J Hregionlymphoproliferative disorders DNA sequences, a multiplex master mix targeting theMonitor and evaluate disease recurrenceconserved framework region 1, framework region 2, or Interpretation TurnaroundSpecimenShippingSpecimen Time Requirements Conditions StabilityAn interpretive report will be5 to 10 1-3 mL ofAmbient orRoom Temp issued indicating whetherbusiness daysperipheral blood inCool; do notup to 72 hoursevidence of clonality wasHeparin, EDTA orfreeze (peripheral2-8 C updetected. The report furtherACD blood or bone provides a summary of themarrow)to 7 days prior 0.25-1 mL of boneto testingtop 5 merged sequences, including the % total reads,marrow in Heparin,Ambient or the rearrangement class andEDTA or ACD frozen on dry ice 500 ng of(isolated DNA)the sequence.previously isolated DNALabPMM Services Catalog 2021|25'