b'MRD TestsTest Name detected at diagnosis. Bioinformatics tools facilitate the detection of these specific sequences present at MRD TRB MRD Clonality Assaylevels up to 1 x 10 -6with sufficient DNA input.Assay Type The assay typically requires a sample taken at diagnosis as well as the post-treatment follow-up samples. If the patient Next-Generation Sequencing (NGS)has previously been tested by LabPMM for TRB clonality, no For Research Use Only diagnostic sample is needed.This test is performed by using the LymphoTrack Assay from Invivoscribe. Data is analyzed using the LymphoTrackIndications for TestingMRD Software (RUO).dentify tumor-specific markers for post-treatment ImonitoringMethod Description Monitor and evaluate disease recurrenceTo track and identify previously detected TRB clonal sequences in post-treatment follow-up samples, a multiplex master mix targeting the V, J and D regionsis used for PCR amplification. Next-generation sequencing of the PCR products is used to identify DNA sequences specific to previously identified clonal rearrangements Interpretation TurnaroundSpecimenShippingSpecimen Time Requirements Conditions StabilityAn interpretive5 to 14 1-3 mL of peripheral blood Ambient or Cool; 2-8 C up report will bebusiness daysin EDTAdo not freezeto 7 days priorissued indicating(peripheral blood orto testingwhether TRB MRD0.25-1 mL of bone marrow bone marrow) was detected in EDTAAmbient or frozen on 700-3500 ng of previously isolated DNA dependingdry ice (isolated DNA)on level of sensitivity requiredLabPMM Services Catalog 2021|45'