b'Gel & Capillary | RUO AssaysGel and CapillaryT-Cell Receptor Gamma Gene Research Use Only (RUO) AssayRearrangement Assay 2.0 T-Cell Receptor Gamma Gene Rearrangement Assay 2.0Assay Use In addition, competitive amplification of all TRG gene rearrangements allows for identification of a quantitative threshold for a positive result This Research Use Only assay identifies T-cell receptor gammaand helps to avoid false positive results. The average size of the TRG (TCRG) chain gene rearrangements and is useful for the identificationgene rearrangement PCR amplicons is 190 nucleotides, with a normal of clonal T-cell populations and evaluation of new research anddistribution of product sizes between 159 and 207 nucleotides. This methods in malignancy studies. protocol leads to improved product formation from formalin-fixed, paraffin-embedded (FFPE) samples compared to other protocols that Summary and Explanation of the Testyield products of 260 nucleotides or larger.This T-Cell Receptor Gamma Gene Rearrangement Assay 2.0 contains a single multiplex master mix which includes primers for all knownSpecimen Requirementsgroups of TCR gamma variable (V) region genes and joining (J)This assay tests extracted and purified genomic DNA (gDNA).region genes involved in rearrangements. In addition, positive and negative controls, as well as a Specimen Control Size Ladder Master Mix are included. PCR products can be analyzed by polyacrylamideReferencesor capillary electrophoresis detection. 1.TC Greiner et al., JMD 4: 137-143 (2002).2. LC Lawnickie et al., JMD 5: 82-87 (2003).Background3. Y Sandberg et al., Leukemia 21: 21 (2007).4. Armand, Marine et al. HemaSphere, 2019;3:3.The human T-cell receptor gamma (TRG, formerly known as TCRG) gene locus on chromosome 7 (7q14) includes 14 V genes belonging to four subgroups, five J segments, and two C genes spread over 200 kilobases. The diversity of this locus has historically complicated PCR-based testing. This multiplex PCR assay represents an improvement over other assays as it can detect the vast majority of TCR gamma gene rearrangements with a single multiplex master mix. This master mix targets all conserved regions within the variable (V) and joining (J) region genes, providing a more comprehensive analysis to include V and J regions that would not be identified with a single V (1-8) and J 1/J 2 primer set.This assay was developed by Invivoscribe.The performance of this assay was reviewed and validated by the EuroClonality/BIOMED-2 Group. 4 Figure Legend: Simple representation of the organization of the T-cell receptor gamma gene on chromosome 7. Black arrows represent the relative positions of primers that target the variable region genes and the downstream joining region gene segments that are involved in rearrangements in T-cell lymphomas. The downstream primers are fluorescently labeled through the incorporation of a 6FAM fluorophore. The amplicon products generated from these rearrangements are detected by capillary electrophoresis.110'