b'Gel & Capillary | RUO AssaysGel and CapillaryTCRB Gene Clonality AssaysResearch Use Only (RUO) AssayTCRB Gene Clonality AssaysAssay Use Specimen RequirementsT-Cell receptor beta (TCRB)gene clonality assays are useful for theThis assay tests extracted and purified genomic DNA (gDNA).identification of T-cell clonality, studying clonal T-cell populations, and evaluation of new research and methods in malignancy studies.ReferencesSummary and Explanation of the Test1.M Bruggemann et al., Leukemia 17: 2283-2289 (2003).Three multiplex master mixes target conserved regions within the2. JJM van Dongen et al., Leukemia 17: 2257-2317 (2003).variable (V), diversity (D), and the joining (J) regions that flank the unique hypervariable, antigen-binding, complementarity determining region 3 (CDR3) of the T-cell receptor beta locus.Tube A contains 23 V primers, six J1 primers, and three J2 primers.Tube B contains 23 V and four J2 primers. Tube C contains two D and 13 J primers. Positive and negative DNA controls, as wellas a Specimen Control Size Ladder Master Mix, are included.PCR products can be analyzed by capillary electrophoresisor heteroduplex analysis. Clonality is indicated, if any one of the master mixes generates clonal products.Background The human T-cell receptor beta (TRB, formerly known as TCRB) gene locus on chromosome 7 (7q34, formerly 7q35) includes 64-67 variable (V) gene segments (belonging to 30 subgroups), two diversity (D) gene segments, and 13 joining (J) gene segments, spread over 685 kilobases. The diversity of this locus has complicated PCR-based testing and extended dependence on Southern blot analysis in many testing centers. However, this standardized multiplex PCR assay detects the vast majority of clonal TRB gene rearrangements using only three multiplex master mixes. 1This assay is based on the EuroClonality/BIOMED-2 Concerted Action BMH4-CT98-3936.Figure Legend: Simplified diagram of a representative rearranged T-cell receptor beta gene showing the approximate placement of the upstream and downstream DNA primers. The numbers of primers and their specificity are listed for Master Mix Tubes A, B, and C.108'