b'Gel & Capillary | RUO AssaysGel and CapillaryBCL2/J HTranslocation AssayResearch Use Only (RUO) AssayBCL2/JTranslocation AssayHAssay Use Specimen RequirementsThis Research Use Only assay identifies BCL2/J Htranslocations. This assay tests extracted and purified genomic DNA (gDNA).Summary and Explanation of the TestReferencesFour master mixes are included in this assay. Three are used to identify1.PAS Evans et al., Leukemia 17: 2298-2301 (2003).translocations in the major breakpoint region (MBR) and minor cluster2. JJM van Dongen et al., Leukemia 17: 2257-2317 (2003).region (mcr) of BCL2. The Specimen Control Size Ladder master mix targets multiple genes and generates a series of amplicons of approximately 100, 200, 300, 400, and 600 base pairs to ensure that the quality and quantity of input DNA is adequate to yield a valid result. This assay includes negative control DNA and positive control DNAs for both the MBR and mcr. PCR products can be analyzed using standard gel electrophoresis with ethidium bromide staining. A BCL2 translocation is indicated if any one of the master mixes generates product(s) within the valid size range.Background BCL2 translocations are reciprocal chromosome exchanges that place the bcl-2 proto-oncogene, located on chromosome 18, under aberrant transcriptional control of the immunoglobulin heavy chain gene, located on chromosome 14. The bcl-2 protein is an antagonist to apoptosis (programmed cell death), a normal process designed to eliminate unneeded and damaged cells during hematopoiesis.Increased expression of the bcl-2 protein leads to an increase in the levels of B-cells in the body.This assay is based on the EuroClonality/BIOMED-2 Concerted Action BMH4-CT98-3936.Figure Legend: Schematic diagram of the IGH-BCL2 t(14;18) translocation showing the BCL2 gene on the left and the Ig heavy chain (IGH) gene on the right. Shown are the relative positions and orientations for the major breakpoint region (MBR) primers, the minor cluster region (mcr) primers, and the J Hprimer, which are included in the 3 BCL2/J Hmaster mix tubes.HHH120'