b'Gel & Capillary | RUO AssaysGel and CapillaryBCL2/J Ht(14;18) Translocation AssayResearch Use Only (RUO) AssayBCL2/Jt(14;18) Translocation AssayHAssay Use Specimen RequirementsThis Research Use Only assay identifies BCL2/J Ht(14;18) translocations. This assay tests extracted and purified genomic DNA (gDNA).Summary and Explanation of the TestReferencesFive master mixes are included in this assay kit. Two master mixes1.MS Lee et al., Science 237: 175-178 (1987).target BCL2 major break point (MBR) translocations and two target2. M Crescenzi et al., Proc. Natl. Acad. Sci. USA 85: 4869-4873 (1988).BCL2 minor cluster region (mcr) translocations. An Amplification Control Master Mix is also included to ensure the quality and quantity of sample DNA. Positive and negative controls are also included. This assay can be run either in a standard or nested assay format. PCR products can be analyzed by standard gel electrophoresis with ethidium bromide staining. A BCL2 translocation is indicated if just one of the 2 ndround master mixes (mixes ending in b) generates product(s) within the valid size range.Background BCL2 translocations are reciprocal chromosome exchanges that place the bcl-2 proto-oncogene, located on chromosome 18, under aberrant transcriptional control of the immunoglobulin heavy chain gene, located on chromosome 14.The bcl-2 protein is an antagonist to apoptosis (programmed cell death), a normal process designed to eliminate unneeded and damaged cells during hematopoiesis.Increased expression of the bcl-2 protein leads to an increase in the levels of B-cells in the body. Figure Legend: Simplified view of the genomic organization of theBCL2 GeneBCL2 and IGH genes on chromosomes 18 and 14, respectively. YellowChromosome 18q21boxes represent the exon regions of the BCL2 gene. Exons of the immunoglobulin heavy chain gene are represented in other colors. The solid black lines represents intron regions, which have been leftHeavy Chain Geneincompletely spliced to assist in demarcation of the exon segments.Chromosome 14q32MBR and mcr type t(14;18) translocations are shown in the lower portions of the figure with the relative positions of primers and the sizeBCL2/IgH Fusion Geneof the amplicons generated from the positive control DNAs indicated. MBR J HBreakpointsBCL2/IgH Fusion Genemcr J HBreakpoints118'