140 Controls | Master Mix & Reagents Invivoscribe 2019 | 141 Controls Reagents & Enzymes Master Mix Controls Amplification Control Master Mix Our Amplification Control master mix targets the HLA-DQa locus and generates a product of 235 basepairs in size from human genomic DNA. This control is available in unlabeled (for Gel Detection) and fluorescence labeled format (for ABI Fluorescence Detection, 6FAM). Specimen Control Size Ladder Our Specimen Control Size Ladder master mix targets four different housekeeping genes producing products of approximately 100, 200, 300, 400, and 600 basepairs in size to ensure that the quality and quantity of the sample DNA is adequate to yield a valid result with the specific assay(s). This master mix is based on the BIOMED-2 Concerted Action BMH4-CT98-3936 from the EuroClonality Group and is available for Gel Detection (unlabeled) or ABI detection (labeled with 6FAM). These master mixes serve as control for many of our DNA assays to ensure that sample DNA is of sufficient quality and integrity to generate a valid result. * These master mixes are general purpose reagents (GPRs). Catalog # Description 2-096-0010 Amplification Control Master Mix - Unlabeled* 2-096-0011 Amplification Control Master Mix - 6FAM* Catalog # Description 2-096-0020 Specimen Control Size Ladder - Unlabeled* 2-096-0021 Specimen Control Size Ladder - 6FAM* Reagents ABI Detection Reagents Reagents for ABI Fluorescence Detection Invivoscribe also offers highly deionized (Hi-Di) Formamide with and without ROX size standards for ABI fluorescence detection with the ABI 310 or 3100 series. Hi-Di Formamide is used to stabilize single strands of denatured PCR amplicons. The ROX size standards are fluorescent labeled DNA standards which cover the 50 to 400 basepair size range. Sizes of the individual standards are: 50, 60, 90, 100, 120, 150, 160, 180, 190, 200, 220, 240, 260, 280, 290, 300, 320, 340, 360, 380, and 400 basepairs. For samples tested on an ABI 310 or 3100 series, we recommend using 10 μL of the Hi-Deionized Formamide with ROX Size Standards mixture for each microliter of PCR product. Please note that the ABI 310 and 3100 series require different concentrations of ROX size standards and the different Hi-Deionized Formamide with ROX Size Standards cannot be used interchangeably. For samples tested on an ABI 3500 series, GeneScan™ 600® LIZ dye Size Standard v2.0 can be purchased from Thermo Fisher Scientific. Ordering Information Catalog # Description 6-098-0051 Hi-Deionized Formamide with ROX Size Standard (ABI 310), 1 mL 6-098-0061 Hi-Deionized Formamide with ROX Size Standard (ABI 3100), 1 mL Available through Thermo Fisher Scientific® : 4408399 GeneScan™ 600 LIZ® dye v2.0 Standard (ABI 3500), 800 reactions Enzymes Taq DNA Polymerase Hot Start Taq DNA Polymerase Taq DNA Polymerase can be used to obtain highly specific and sensitive PCR amplification products. This enzyme has been proven to minimize extension of non-specifically bound primers. Obtain reliable results by using the gold standard of hot start polymerases for robust performance. Ordering Information Catalog # Description 6-097-0100 Taq DNA Polymerase 1000 U, 5 U/μL Note: This product is for sale and use in the European Economic Area only. It is not to be resold or transferred to another party. See also Notice on page 126. For research use only (RUO), not for diagnostic procedures.