128 Controls | DNA Controls Invivoscribe 2019 | 129 Controls DNA Controls DNA Controls Tissue DNA Cell Line DNA Quick Reference for DNA Controls The vast majority of our high-quality DNA controls, including sensitivity controls and panels, are supplied in aliquots of 100 μL and are adjusted to a final concentration of 200 μg/mL in 1/10 TE (1 mM Tris- HCl (pH 8.0), 0.1 mM EDTA). Positive for Immunoglobulin Rearrangements Mutations Translocations T-Cell Receptor Gene Rearrangements IGH IGK IGL IGHV SHM IGH-CCND1 ** IGH-BCL2 TRB TRG TRD IVS-0001 u IVS-0004 u u IVS-0007 u u u IVS-0008* m u u IVS-0009 u u IVS-0010 u u u u IVS-0013 u u u IVS-0019 u u IVS-0021 u u u u IVS-0024 u u IVS-0029 u u u IVS-0030 ‡ u u u u IVS-0031 u u u IVS-P002 u * This control does not contain a complete IGH VH-JH rearrangement and may only be suitable for IGH DH-JH rearrangements. ** IGH-CCND1 was previously referred to as BCL1/JH Standard Concentrations The vast majority of our high-quality DNA controls, including sensitivity controls and panels, are supplied in aliquots of 100 μL and are adjusted to a final concentration of 200 μg/mL in 1/10 TE (1 mM Tris- HCl (pH 8.0), 0.1 mM EDTA). This diluent provides sufficient buffering capacity and EDTA to protect the DNA without interfering with the Mg2+ concentrations required for robust amplification reactions. IVS-0000 Polyclonal Control DNA Tissue DNA controls are extracted from normal, disease-free tissue and are tested extensively to ensure quality and reproducibility of your test results. IVS-0000 Polyclonal Control DNA consists of genomic DNA isolated from the tissue of normal human tonsils. This control represents an excellent negative control for gene rearrangements, chromosome translocations, and mutation tests and is included in all of our PCR DNA-based assay kits. This DNA is supplied at a volume of 100 μL and at a concentration of 200 μg/mL. Catalog # Description 4-092-0010 IVS-0000 Polyclonal Control DNA* IVS-0001 Clonal Control DNA IVS-0001 Clonal Control DNA can be used as a positive control for: Gene Rearrangements: n/c Chromosome Translocations: IGH-BCL2 t(14;18) mcr Mutations: n/c Catalog # Description 4-088-0010 100% IVS-0001 Clonal Control DNA* IVS-0007 Clonal Control DNA IVS-0007 Clonal Control DNA can be used as a positive control for: Gene Rearrangements: IGH, IGK, IGL Chromosome Translocations: IGH-BCL2 t(14;18) Mbr Mutations: n/c This control is also available as several ready-to-use dilutions into a standard negative control as listed in the table below. Catalog # Description 4-088-0370 100% IVS-0007 Clonal Control DNA* 4-088-0390 20% IVS-0007 Clonal Control DNA 4-088-0400 10% IVS-0007 Clonal Control DNA 4-088-0410 5% IVS-0007 Clonal Control DNA 4-088-0420 1% IVS-0007 Clonal Control DNA IVS-0004 Clonal Control DNA IVS-0004 Clonal Control DNA can be used as a positive control for: Gene Rearrangements: TRB, TRG Chromosome Translocations: n/c Mutations: n/c This control is available as several ready-to-use dilutions generated by diluting the positive control volume to volume (v/v) into DNA characterized as negative. Catalog # Description 4-088-0190 100% IVS-0004 Clonal Control DNA* 4-088-0210 20% IVS-0004 Clonal Control DNA 4-088-0220 10% IVS-0004 Clonal Control DNA 4-088-0230 5% IVS-0004 Clonal Control DNA Reliable Positive Controls Cell Line DNA controls are extracted from established cell lines grown under cell culture conditions recommended by the supplier. Our controls are tested extensively to ensure quality and reproducibility of your test results. Please note, these controls are for qualitative use only. Note: n/c is used to indicate that the control has not been fully characterized; there may be additional rearrangements, translocations or mutations associated with the control. Standard Concentrations The majority of our high-quality DNA controls, including our sensitivity panels, are supplied in aliquots of 100 μL and are adjusted to a final concentration of 200 μg/mL in 1/10 TE (1 mM Tris- HCl (pH 8.0), 0.1 mM EDTA). This diluent provides sufficient buffering capacity and EDTA to protect the DNA controls without interfering with the Mg2+ concentrations required for robust amplification reactions. DNA dilutions are diluted volume to volume (v/v) in our negative control DNA, IVS-0000 Polyclonal Control DNA. *  These controls are general purpose reagents (GPRs). All others are research use only (RUO). Every laboratory needs suitable controls (positive and negative) for sensitivity and proficiency testing, as well as for troubleshooting. Since patient samples cannot serve as true controls (due to a lack of characterization and inter-sample variability), Invivoscribe offers a multitude of high quality, reliable DNA controls manufactured under cGMP conditions. These controls can be used for all assays targeting B- and T-cell antigen receptor loci, as well as IGH-BCL2, BCR-ABL1, and PML-RARa chromosome translocations. ‡ These controls can be used as SHM positive controls with ≥2% mutational rates compared to the germline sequence. u Gene rearrangement m Partial IGH DH-JH rearrangement