134 Controls | RNA Controls Controls RNA Controls Invivoscribe 2019 | 135 RNA Controls Quick Reference for RNA Controls Cell Line RNA Reliable Assay Controls Our RNA controls are extracted from well characterized cell lines grown under standard and carefully controlled culture conditions. The controls are tested to ensure linearity and reproducible results. Since this RNA is extracted from cell lines, these controls can be used with any of the standard housekeeping genes. Standard Concentrations Each RNA single control tube (as separate control tube, RNA sensitivity panel and proficiency panel) is supplied in aliquots of 100 μL at a final concentration of 400 μg/mL in water. Each BCR/ABL RNA dilution set member is supplied in aliquots of 50 μL at a final concentration of 400 μg/mL in water. To ensure maximum stability, the dilution set should be stored at -65 °C to -85 °C and the number of freeze-thaw cycles should be kept to a minimum. RNAs positive for chromosome translocations Chromosome Translocation Clonal Control RNA Chromosome Translocation Clonal Control RNA BCR-ABL1 t(9;22) p210 e13a2 (b2a2) IVS-0003 CBFB-MYH11 inv(16) IVS-0015 BCR-ABL1 t(9;22) p210 e14a2 (b3a2) IVS-0011 E2A-PBX1 t(1;19)(q23;p13) IVS-0002 BCR-ABL1 t(9;22) p190 e1a2 IVS-0032 PML-RARA t(15;17)(q22;q11) IVS-0020 RNAs negative for chromosome translocations IVS-0035 can be used as a negative control for our BCR/ABL t(9;22) and PML/RARA t(15;17) Translocation Assays. IVS-0035 may be used as a negative control for other chromosome translocation assays, or diluents for other chromosome translocation positive controls. Please do not hesitate to contact us at sales@invivoscribe.com so we can evaluate whether this control may work for your testing needs. Reliable Positive and Negative Controls Cell Line RNA controls are extracted from established cell lines grown under cell culture conditions recommended by the supplier. Our controls are tested extensively to ensure quality and reproducibility of your test results. Please note, these controls are for qualitative use only. Standard Concentrations Our GMP-manufactured high-quality RNA controls, including sensitivity controls and proficiency panel samples, are supplied in aliquots of 100 μL and are adjusted to a final concentration of 400 μg/mL in RNase-free glass-distilled water. The pH of distilled water is slightly acidic; this protects the RNA from hydrolysis. RNA dilutions are diluted volume to volume in our negative control RNA, IVS-0035 Clonal Control RNA. IVS-0002 Clonal Control RNA IVS-0002 Clonal Control RNA can be used as a positive control for the chromosome translocation: E2A-PBX1 t(1;19) (q23;p13). Catalog # Description 4-089-0100 IVS-0002 Clonal Control RNA* IVS-0035 Clonal Control RNA IVS-0035 Clonal Control RNA can be used as a negative control for our BCR/ABL1 t(9;22) and PML/RARa t(15;17) Translocation Assays. Catalog # Description 4-089-3070 IVS-0035 Clonal Control RNA* IVS-0015 Clonal Control RNA IVS-0015 Clonal Control RNA can be used as a positive control for the chromosome translocation: CBFB-MYH11 inv(16). This control is also available as several ready-to-use dilutions into a standard negative control as listed in the table below. Catalog # Description 4-089-1270 IVS-0015 Clonal Control RNA* IVS-0003 Clonal Control RNA IVS-0003 Clonal Control RNA can be used as a positive control for the chromosome translocation:  BCR-ABL1 t(9;22) p210 e13a2 (b2a2). This control is also available as several ready-to-use dilutions into a standard negative control as listed in the table below. Catalog # Description 4-089-0190 IVS-0003 Clonal Control RNA* 4-089-0200 10-1 IVS-0003 Clonal Control RNA 4-089-0210 10-2 IVS-0003 Clonal Control RNA 4-089-0220 10-3 IVS-0003 Clonal Control RNA 4-089-0230 10-4 IVS-0003 Clonal Control RNA 4-089-0240 10-5 IVS-0003 Clonal Control RNA 4-089-0250 10-6 IVS-0003 Clonal Control RNA IVS-0020 Clonal Control RNA IVS-0020 Clonal Control RNA can be used as a positive control for the chromosome translocation: PML-RARA t(15;17) L-Form. This control is also available as several ready-to-use dilutions into a standard negative control as listed in the table below. Catalog # Description 4-089-1720 IVS-0020 Clonal Control RNA* 4-089-1730 10-1 IVS-0020 Clonal Control RNA 4-089-1740 10-2 IVS-0020 Clonal Control RNA 4-089-1750 10-3 IVS-0020 Clonal Control RNA 4-089-1760 10-4 IVS-0020 Clonal Control RNA IVS-0011 Clonal Control RNA IVS-0011 Clonal Control RNA can be used as a positive control for the chromosome translocation:  BCR-ABL1 t(9;22) p210 e14a2 (b3a2). Catalog # Description 4-089-0910 IVS-0011 Clonal Control RNA* 4-089-0920 10-1 IVS-0011 Clonal Control RNA 4-089-0930 10-2 IVS-0011 Clonal Control RNA 4-089-0940 10-3 IVS-0011 Clonal Control RNA 4-089-0950 10-4 IVS-0011 Clonal Control RNA 4-089-0960 10-5 IVS-0011 Clonal Control RNA IVS-0032 Clonal Control RNA IVS-0032 Clonal Control RNA can be used as a positive control for the chromosome translocation: BCR-ABL1 t(9;22) p190 e1a2. This control is also available as several ready-to-use dilutions into a standard negative control as listed in the table below. Catalog # Description 4-089-2800 IVS-0032 Clonal Control RNA* 4-089-2810 10-1 IVS-0032 Clonal Control RNA 4-089-2820 10-2 IVS-0032 Clonal Control RNA 4-089-2830 10-3 IVS-0032 Clonal Control RNA 4-089-2840 10-4 IVS-0032 Clonal Control RNA 4-089-2850 10-5 IVS-0032 Clonal Control RNA 4-089-2860 10-6 IVS-0032 Clonal Control RNA *  These controls are general purpose reagents (GPRs). All others are research use only (RUO).