138 Controls | Control Panels Invivoscribe 2019 | 139 Controls Control Panels Proficiency Panel for BCR-ABL1 t(9;22) This 10 member panel consists of 100% clonal control RNA extracted from three BCR-ABL1 positive cell lines as well as 10-2 (1:100) and 10-4 (1:10,000) dilutions (v/v) of these positive RNAs diluted into a normal (BCR-ABL1 negative) control RNA, IVS-0035 Clonal Control RNA. A sample of 100% IVS-0035 Clonal Control RNA is also included. All three cell lines (IVS-0003, IVS-0011, and IVS-0032) carry a t(9;22) translocation. One of the cell lines, IVS-0032, encodes for p190-type (ALL-associated) transcript with e1a2 junctions. The other two cell lines both encode for p210-type (CML-associated) transcripts. One of the p210-type translocations, IVS-0003, harbors a e13a2 (b2a2) junction and the other, IVS-0011, harbors a e14a2 (b3a2) junction. This proficiency panel is used to validate tests that identify BCR-ABL1 t(9;22) translocations and is designed to be used with the BCR/ABL t(9;22) Translocation Assay Kits. Ordering Information Catalog # Description 4-310-0100 Proficiency Panel for the BCR/ABL t(9;22) Translocation Assay DNA and RNA Sensitivity Panels Control Panels T Cell Receptor Gamma Mix 1 Panel 1 Panel 3 Panel 2 Panel 7 Panel 6 Panel 5 Panel 4 100% IVS-0009 20% IVS-0009 30% IVS-0009 IVS-0000 1% IVS-0009 5% IVS-0009 10% IVS-0009 T Cell Receptor Gamma Mix 2 Panel 1 Panel 3 Panel 2 Panel 7 Panel 6 Panel 5 Panel 4 100% IVS-0009 20% IVS-0009 30% IVS-0009 IVS-0000 1% IVS-0009 5% IVS-0009 10% IVS-0009 DNA Sensitivity Panels DNA sensitivity panels are 6 member panels that consist of 100% clonal DNA extracted from a positive control cell line and 30%, 20%, 10%, 5%, and 1% dilutions of the positive clonal DNA diluted (v/v) into our standard negative control DNA, IVS-0000 Polyclonal Control DNA. Each tube contains 100 μL of DNA at a concentration of 200 μg/mL in 1/10 TE buffer (1 mM Tris-HCl pH 8.0, 0.1 mM EDTA). This diluent provides sufficient buffering capacity and EDTA to protect the DNA controls without interfering with the Mg2+ concentrations required for robust amplification reactions. Please note, these controls are for qualitative use only. RNA Sensitivity Panels RNA sensitivity panels are 7 member panels that consist of 100% clonal RNA extracted from a positive control cell line and 10-1 , 10-2 , 10-3 , 10-4 , 10- 5 , and 10-6 (1:10 - 1:1 000 000) dilutions of the positive clonal RNA diluted (v/v) into our standard negative control RNA, IVS-0035 Clonal Control RNA. Each tube contains 100 μL of RNA at 400 μg/mL in RNase-free glass-distilled water. The pH of distilled water is slightly acidic thereby protecting the RNA from hydrolysis. Please note, these controls are for qualitative use only. This data was generated testing a Sensitivity Panel for IVS-0009 Clonal Control DNA using the master mixes listed. PCR products were run on an ABI 3130xL capillary electrophoresis instrument for differential fluorescence detection and data analyses. Panel 7 shows the polyclonal Gaussian distributions expected from our negative control IVS-0000 Polyclonal Control DNA. Data in the other panels are tests of positive control samples at the dilutions indicated. Clonal peaks (highlighted) are clearly evident in all of the positive sample panels. RNA Sensitivity Panels Catalog # Description Can be used as a positive control for: 4-087-0030 Sensitivity Panel for IVS-0003 Clonal Control RNA BCR-ABL1 t(9;22) p210 e13a2 (b2a2) 4-087-0110 Sensitivity Panel for IVS-0011 Clonal Control RNA BCR-ABL1 t(9;22) p210 e14a2 (b3a2) 4-087-0150 Sensitivity Panel for IVS-0015 Clonal Control RNA CBFB/MYH11 inv16 4-087-0200 Sensitivity Panel for IVS-0020 Clonal Control RNA PML-RARA t(15;17) L-form 4-087-0320 Sensitivity Panel for IVS-0032 Clonal Control RNA BCR-ABL1 t(9;22) p190 e1a2 DNA Sensitivity Panels Catalog # Description Can be used as a positive control for: 4-086-0040 Sensitivity Panel for IVS-0004 Clonal Control DNA TRB, TRG 4-086-0070 Sensitivity Panel for IVS-0007 Clonal Control DNA IGH, IGK, IGL, IGH-BCL2 t(14;18) Mbr 4-086-0090 Sensitivity Panel for IVS-0009 Clonal Control DNA TRB, TRG 4-086-0100 Sensitivity Panel for IVS-0010 Clonal Control DNA IGH, IGK, IGL, IGH-CCND1 t(11;14) 4-086-0190 Sensitivity Panel for IVS-0019 Clonal Control DNA IGH, IGK 4-086-0210 Sensitivity Panel for IVS-0021 Clonal Control DNA TRB, TRD, TRG 4-086-0300 Sensitivity Panel for IVS-0030 Clonal Control DNA IGH, IGK, IGH-BCL2 t(14;18) Mbr RNA Proficiency Panel Qty Description Chromosome Translocation 1 IVS-0003 Clonal Control RNA 10-2 IVS-0003 Clonal Control RNA 10-4 IVS-0003 Clonal Control RNA BCR-ABL1 p210 e13a2 (b2a2) 1 IVS-0011 Clonal Control RNA 10-2 IVS-0011 Clonal Control RNA 10-4 IVS-0011 Clonal Control RNA BCR-ABL1 p210 e14a2 (b3a2) 1 IVS-0032 Clonal Control RNA 10-2 IVS-0032 Clonal Control RNA 10-4 IVS-0032 Clonal Control RNA BCR-ABL1 p190 e1a2 1 IVS-0035 Clonal Control RNA BCR-ABL1 Negative These products are research use only (RUO), not for diagnostic procedures. These products are research use only (RUO), not for diagnostic procedures.